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AB5541

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

Chemicon®, from chicken

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Synonym(s):
GFAP
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, rat, bovine, pig

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

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MAB5628MAB3402MAB360
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

UniProt accession no.

P14136

UniProt accession no.

P14136

UniProt accession no.

F1RR02

UniProt accession no.

P14136

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

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General description

Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Specificity

Expected to cross-react with bovine and porcine.
Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Other species have not yet been tested.

Immunogen

Purified bovine GFAP.

Application

Immunohistochemistry(paraffin):
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.

Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.

Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.

Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.

Optimal working dilutions must be determined by the end user.

STAINING PATTERN:

The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
This Anti-Glial Fibrillary Acidic Protein Antibody is validated for use in IC, IH, IH(P), WB for the detection of GFAP.

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.

Target description

55 kDa

Linkage

Replaces: 04-1031; 04-1062

Physical form

Purified chicken polyclonal in buffer containing PBS containing 0.02% sodium azide.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Astrocytes, astrocytomas, neural stem cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Protocols

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

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