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AB5898

Sigma-Aldrich

Anti-Protein Gene Product 9.5 Antibody

serum, Chemicon®

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Synonym(s):
PGP 9.5, UCH-L1
eCl@ss:
32160702
NACRES:
NA.41

biological source

guinea pig

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UCHL1(7345)
mouse ... Uchl1(22223)
rat ... Uchl1(29545)

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This Item
AB5925NE1013SAB2501667
antibody form

serum

antibody form

serum

antibody form

serum

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, rat, mouse

species reactivity

mouse, human, pig, rat

species reactivity

porcine, human, rat, mouse

species reactivity

rat, human, bovine, canine, pig

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

-

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

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Specificity

Recognizes Protein Gene Product 9.5 (PGP9.5).

Immunogen

A 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5.

Application

Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.
Immunohistochemistry: 1:500.

Optimal working dilutions must be determined by end user.

blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Physical form

Liquid. Contains 0.05% sodium azide.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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M Bagyánszki et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 23(6), e237-e248 (2011-04-08)
There are indications that alterations in the nitric oxide (NO) system of relaxation mediate gastrointestinal motor disturbances induced by chronic alcohol consumption (CAC). As CAC is known to inhibit the motility of the mouse small intestine, we investigated in this
Nociceptin effect on intestinal motility depends on opioid-receptor like-1 receptors and nitric oxide synthase co-localization.
Sibaev, A; Fichna, J; Saur, D; Yuece, B; Timmermans, JP; Storr, M
World journal of gastrointestinal pharmacology and therapeutics null
Rebecca S Hornung et al.
Molecular pain, 18, 17448069211069255-17448069211069255 (2022-01-19)
Orofacial pain disorders are predominately experienced by women. Progesterone, a major ovarian hormone, is neuroprotective and antinociceptive. We recently reported that progesterone attenuates estrogen-exacerbated orofacial pain behaviors, yet it remains unclear what anatomical substrate underlies progesterone's activity in the trigeminal
Allison Doyle Brackley et al.
The Journal of biological chemistry, 292(21), 8762-8772 (2017-04-07)
μ-Opioid receptor (MOR) agonists are often used to treat severe pain but can result in adverse side effects. To circumvent systemic side effects, targeting peripheral opioid receptors is an attractive alternative treatment for severe pain. Activation of the δ-opioid receptor
Birol Yüce et al.
Gastroenterology, 133(2), 574-586 (2007-08-08)
Nociceptin is the endogenous agonist of the "orphan" opioid receptor-1 (ORL-1). We investigated whether activation of the ORL-1 receptor influences smooth muscle contractility and enteric neurotransmission within ascending myenteric reflex pathways of rats. Reverse transcriptase polymerase chain reaction was performed

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