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Key Documents

AB6019

Sigma-Aldrich

Anti-Thymosin beta-4 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

thymosin beta 4, X-linked, prothymosin beta-4, thymosin, beta 4, thymosin, beta 4, X chromosome, T beta-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_066932

UniProt accession no.

P62328

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TMSB4X(7114)

General description

Thymosin beta-4 is the most prevalent of the cytoplasmic G-actin binding proteins belonging to the highly conserved thymosin beta family. Known to bind actin monomers, Thymosin beta-4 sequesters G-actin in a manner that prevents actin polymerization. Thymosin beta-4 plays several important roles including induction of tissue remodeling, wound healing in both cell and tissue injuries, and cell differentiation via mechanisms that have yet to be fully elucidated. It is involved in the regulation of the cytoskeleton, and thereby influences an array of cellular processes. Within the brain, Thymosin beta-4 is associated with the neurite extension and migration of neurons. Expression has been observed in various hemopoietic and lymphoid malignant cell lines.

Specificity

Th antibody recognizes the internal domain of Thymosin beta-4.

Immunogen

GST-tagged recombinant protein corresponding to the internal domain of human Thymosin beta-4.

Application

Anti-Thymosin beta-4 Antibody is an antibody against Thymosin beta-4 for use in WB & IC.
Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected Thymosin beta-4 on 10 µg of recombinant Thymosin beta-4.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Thymosin beta-4 in NIH/3T3, HeLa, and A431 cells.

Quality

Evaluated by Western Blot in recombinant Thymosin beta-4.

Western Blot Analysis: 1 µg/mL of the antibody detected Thymosin beta-4 on 10 µg of recombinant Thymosin beta-4.

Target description

~ 33kDa observed

Analysis Note

Control
Recombinant Thymosin beta-4

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Vinay Saunders et al.
PloS one, 13(11), e0207248-e0207248 (2018-11-10)
The objective of this study was to investigate the expression and localisation of thymosin β4 (Tβ4) in the developing human heart. Tβ4 is a cardioprotective protein which may have therapeutic potential. While Tβ4 is an endogenously produced protein with known
Krishnanand Padmanabhan et al.
Development (Cambridge, England), 147(23) (2020-12-15)
Planar cell polarity (PCP) is essential for tissue morphogenesis and homeostasis; however, the mechanisms that orchestrate the cell shape and packing dynamics required to establish PCP are poorly understood. Here, we identified a major role for the globular (G)-actin-binding protein
Jong-Ho Kim et al.
International journal of molecular sciences, 21(6) (2020-04-05)
Thymosin β4 (Tβ4) is a G-actin sequestering protein that contributes to diverse cellular activities, such as migration and angiogenesis. In this study, the beneficial effects of combined cell therapy with Tβ4 and human adipose-derived stem cells (hASCs) in a mouse

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