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ABN278

Sigma-Aldrich

Anti-Prospero homeobox protein 1/PROX1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), fish (based on 100% sequence homology), chicken (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Prox1(19130)

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This Item
MAB5652AB5475SAB5700082
vibrant-m

MAB5652

Anti-Prox1 Antibody, clone 5G10

vibrant-m

AB5475

Anti-Prox 1 Antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

serum

antibody form

affinity isolated antibody

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

species reactivity

mouse, rat

species reactivity

human, mouse, chicken, lizard, rat

species reactivity

rat, mouse, zebrafish

species reactivity

human, rat, mouse

Gene Information

mouse ... Prox1(19130)

Gene Information

mouse ... Prox1(19130)

Gene Information

human ... PROX1(5629)

Gene Information

human ... PROX1(5629)

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

western blot: 1:500-1:2000

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General description

PROX1 (Prospero homeobox protein 1), the homolog of Drosophila Prospero, is a homeobox-containing transcription factor that binds and functions as a co-receptor of liver receptor homolog 1 (LRH1/NR5A2). It is a specific marker for lymphatic endothelial cells, as well as hepatoblasts and hepatocytes. Human Prox1 shares 97% amino acid sequence identity with mouse and rat Prox1.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Prospero homeobox protein 1/PROX1.

Application

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Prospero homeobox protein 1/PROX1 in mouse E16 embryo thalamus tissues (Prof. Samual Pleasure, University of California, San Francisco.).

Alexa Fluor is a registered trademark of Life Technologies, Inc.
This Anti-Prospero homeobox protein 1/PROX1 Antibody is validated for use in western blotting & IHC for the detection of Prospero homeobox protein 1/PROX1.

Quality

Evaluated by Western Blotting in HEK293 nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Prospero homeobox protein 1/PROX1 in 10 µg of HEK293 nuclear extract.

Target description

~95 kDa observed. Uniprot describes a molecular weight at 83 kDa However, this protein may be observed between 80-110 kDa due to sumoylation and/or alternative start sites (Pan, M. R., et al. (2009). J Cell Sci. 122(Pt18):3358-3361.; Azuma, K., et al. (2011). Genes Cells. 16(11):1063-1070.).

Linkage

Replaces: AB5475

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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