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ABN30

Sigma-Aldrich

Anti-Aromatase Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Cytochrome P450 19A1, Aromatase, CYPXIX, Cytochrome P-450AROM, Estrogen synthase
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, pig

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CYP19A1(1588)

General description

Human Aromatase is a membrane-bound enzyme that is localized on the endoplasmic reticulum. While it is found in gonads and adrenal glands where it converts androgens into estrogens in a classical endocrine manner, it is also expressed in various tissues where it regulates local estrogen levels or estrogen/androgen ratios in an intracrine manner. Human Aromatase is known to have two major forms, characterized by one amino acid difference at amino acid position 264 [arginine (264R) and cysteine (264C)]. Abnormal Aromatase hyperactivity is a known cause for Aromatase excess syndrome (AES), which is characterized by excess levels of estrogen, heterosexual precocity in adolescent males and isosexual precocity in adolescent females. On the other hand, Aromatase deficiency (AROD) in Aromatase-deficient (ArKO) mice leads to increased adiposity, an inability to synthesize estrogen, birth defects, and genital-related developmental disorders. It is also reported to play a role in a complex immune-neuro-endocrine network management system in murine cysticercosis.

Specificity

Other homolgoies: Rat and Mouse (94% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human Aromatase.

Application

Anti-Aromatase Antibody is an antibody against Aromatase for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Western Blot Analysis: 1 µg/mL from a representative lot detected Aromatase in 10 µg of human testis.

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Aromatase in 10 µg of human placenta tissue lysate. Tween was not used in the Western Blot.

Target description

~58 kDa observed. UniProtKB/Swiss-Prot P11511 (CP19A_HUMAN) states a molecular weight of ~58 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human placenta tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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