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ABN469

Sigma-Aldrich

Anti-DISC1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
DISC1 Antibody, Disrupted in schizophrenia 1 protein, KIAA0457
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DISC1(27185)

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This Item
ABN308SAB2500312SAB4200622
Anti-DISC1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN469

Anti-DISC1 Antibody

Anti-DISC1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN308

Anti-DISC1 Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

-

species reactivity

mouse

species reactivity

rat, mouse, human

species reactivity

human

species reactivity

human, rat

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: suitable

technique(s)

immunoblotting: 1-2 μg/mL using whole extracts of rat brain P2 fraction or HEK-293T cells over-expressing human DISC1, immunoprecipitation (IP): 2.5-5.0 μg using extracts of mouse brain.

UniProt accession no.

Q9NRI5

UniProt accession no.

Q9NRI5

UniProt accession no.

Q9NRI5

UniProt accession no.

Q9NRI5

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General description

Disrupted in schizophrenia 1 protein (DISC1) is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 may play a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. DISC1 is shown to inhibit the activation of AKT-mTOR signaling upon interaction with CCDC88A. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Additionally, DISC1 is suggested to regulate the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. DISC1 has been shown to participates in the Wnt-mediated neural progenitor proliferation as a positive regulator and may have a role, together with PCNT, in the microtubule network formation.
DICS1 is highly expressed in the dentate gyrus of the hippocampus. It is also expressed in the temporal and parahippocampal cortices and cells of the white matter. Expression rises within the dentate gyrus and temporal cortex from the neonatal period to infancy, declines markedly in adolescence, and declines further with aging. Uniprot describes 8 isoforms ranging between ~37 kDa and ~94 kDa.

Specificity

This antibody recognizes the N-terminus of DISC1.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human DISC1.

Application

Anti-DISC1 Antibody is a highly specific rabbit polyclonal antibody, that targets Disc1 & has been tested in western blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DISC1 in 15 µg of NIH/3T3 cell lysate.

Target description

~95 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 8 isoforms ranging between ~37 kDa and ~94 kDa

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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