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ABN910

Sigma-Aldrich

Anti-Aquaporin 4

from rabbit, purified by affinity chromatography

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Synonym(s):
AQP-4, Mercurial-insensitive water channel, MIWC
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... AQP4(361)
mouse ... Aqp4(11829)
rat ... Aqp4(25293)

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This Item
MABN2471AB2218AB3594
Anti-Aquaporin 4 from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN910

Anti-Aquaporin 4

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

mECD, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, mouse, rat

species reactivity

human, rat, mouse

species reactivity

human, mouse, rat

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

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General description

Aquaporin-4 (UniProt: P47863; also known as AQP-4, Mercurial-insensitive water channel, MIWC) is encoded by the Aqp4 gene (Gene ID: 25293) in rat. Aquaporin-4 is a multi-pass membrane homotetrameric protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).

Specificity

This rabbit polyclonal antiody detects Aquaporin-4. It targets an epitope with in 75 amino acids from the C-terminal.

Immunogen

Epitope: C-terminus
GST/HIs-taged recombinant fragment corresponding to 75 amino acids from the C-terminal region of rat Aquaporin-4.

Application

Anti-Aquaporin 4, Cat. No. ABN910, is a highly specific rabbit polyclonal antibody that targets Aquaporin 4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution from a representative lot detected Aquaporin 4 in rat cerebral cortex tissue.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Aquaporin 4 in human brain tissue lysate.
Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry (Paraffin) in mouse cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis : A 1:1,000 dilution of this antibody detected Aquaporin 4 in mouse cerebral cortex tissue.

Target description

~34.0 kDa observed; 34.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl (pH 7.4) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Rachel Britton et al.
Brain and behavior, 12(9), e2736-e2736 (2022-08-17)
Increasing age is the number one risk factor for developing cognitive decline and neurodegenerative disease. Aged humans and mice exhibit numerous molecular changes that contribute to a decline in cognitive function and increased risk of developing age-associated diseases. Here, we

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