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ABN998

Sigma-Aldrich

Anti-phospho PSD95 Antibody (Thr19)

from rabbit, purified by affinity chromatography

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Synonym(s):
Disks large homolog 4, Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pThr19 )

Gene Information

human ... DLG4(1742)

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Anti-PSD95 Antibody, clone 1A8.1 clone 1A8.1, from mouse

MABN1190

Anti-PSD95 Antibody, clone 1A8.1

Anti-SAPAP1 Antibody from rabbit, purified by affinity chromatography

ABN309

Anti-SAPAP1 Antibody

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable, inhibition assay: suitable (peptide)

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:200- 1:500

technique(s)

immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

UniProt accession no.

P78352

UniProt accession no.

P78352

UniProt accession no.

P78352

UniProt accession no.

O14490

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General description

PSD95 (postsynaptic density95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.

Immunogen

Epitope: Near N-Terminus
KLH-conjugated linear peptide corresponding to human phospho PSD95 (Thr19) near the N-Terminus.

Application

Anti-phospho PSD95 Antibody (Thr19) is an antibody against phospho PSD95 (Thr19) for use in Western Blotting, Peptide Inhibition Assay, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected phospho PSD95 (Thr19) in human cerebellum, human cerebral cortex, and human thalamus tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in human fetal brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected phospho PSD95 (Thr19) in 10 µg of human fetal brain tissue lysate.

Target description

~80 kDa observed. The calculated molecular weight is 80 kDa, however phospho PSD95 (Thr19) has been shown as a ~80-95 kDa band in some western blots (Nelson, C.D., et al. (2013). J. of Neuroscience. 33(29):12122-12135).

Physical form

Affinity
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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