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ABS2206

Sigma-Aldrich

Anti-phospho-Ubiquitin (Ser57)

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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

observed mol wt ~28 kDa

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse, chimpanzee, hamster, rat

packaging

antibody small pack of 100 μg

technique(s)

affinity binding assay: suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal half

Protein ID accession no.

UniProt accession no.

shipped in

2-8°C

target post-translational modification

phosphorylation (pSer67)

Gene Information

human ... UBB(7314)

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This Item
MABS486U5379662099
vibrant-m

ABS2206

Anti-phospho-Ubiquitin (Ser57)

vibrant-m

662099

Anti-Ubiquitin Rabbit pAb

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

-

antibody form

purified antibody

antibody form

purified antibody

antibody form

whole antiserum

antibody form

serum

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

UniProt accession no.

P0CG47

UniProt accession no.

P0CG47, P0CG48

UniProt accession no.

P0CG47

UniProt accession no.

-

clone

polyclonal

clone

2G7B8, monoclonal

clone

polyclonal

clone

polyclonal

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General description

Ubiquitin (Ub) is initially produced as a 229 amino acids Polyubiquitin-B (UniProt: P0CG47) precursor protein encoded by the UBB gene (Gene ID: 7314) or a 685 amino acids Polyubiquitin-C precursor protein (UniProt: P0CG48) encoded by the UBC gene (Gene ID: 7316) in human. Ub exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Ub is linked covalently via its carboxyl terminus (Gly76) to usually Lys residues in target proteins. In a given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyUb chain, Ub molecules can be linked through one of the seven Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. For example, lysine 6-linked may be involved in DNA repair; lysine 11-linked is involved in endoplasmic reticulum-associated degradation (ERAD) and in cell-cycle regulation, and lysine 29-linked is involved in lysosomal degradation. Lysine 48-linked chains mark proteins for proteasomal degradation, while lysine 63-linked chains are involved in endocytosis, DNA-damage responses, and in signaling leading to activation of the transcription factor NF-kappa-B. Ubiquitin undergoes phosphorylation at the serine 57 by a ubiquitin kinase and this phosphorylation is an important modifier of ubiquitin function, particularly in response to proteotoxic stress. This phosphorylation may also be a deciding factor whether ubiquitin is recycled or degraded during multi-vesicular body sorting on endosomes. (Ref.: Hepowit, NL., et al (2020). eLife 9; e58155; Lee, S., et al. (2017) eLife. 6; e29176).

Specificity

This rabbit polyclonal antibody specifically detects Ubiquitin phosphorylated on serine 57.

Immunogen

KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 57 of human Ubiquitin.

Application

Quality Control Testing

Evaluated by Western Blotting with a construct containing GST-tagged recombinant fragment of Ubiquitin phosphorylated on serine 57.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected a construct containing GST-tagged recombinant Ubiquitin phosphorylated on serine 57, but did not react with non-phosphorylated construct. (Phospho-Ubiquitin construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine).

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound phospho-Ubiquitin (ser57) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-phospho-Ubiquitin (Ser57), Cat. No. ABS2206, is a rabbit polyclonal antibody that detects phospho-Ubiquitin (Ser57) and is tested for use in Affinity Binding Assay and Western Blotting.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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