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ABT135

Sigma-Aldrich

Anti-phospho-FAK (Tyr397) Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Focal adhesion kinase 1, FADK 1, Protein-tyrosine kinase 2, pp125FAK
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat

species reactivity (predicted by homology)

human (based on 100% sequence homology), mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

cell based assay: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr397)

Gene Information

human ... PTK2(5747)

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SAB4504181SAB4301455SAB4502500
Anti-phospho-FAK (Tyr397) Antibody from rabbit, purified by affinity chromatography

ABT135

Anti-phospho-FAK (Tyr397) Antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Gene Information

human ... PTK2(5747)

Gene Information

human ... PTK2(5747)

Gene Information

human ... PTK2(5747)

Gene Information

human ... PTK2(5747)

species reactivity

rat

species reactivity

mouse, human, rat

species reactivity

rat, mouse, human

species reactivity

mouse, rat, human

technique(s)

cell based assay: suitable, western blot: suitable

technique(s)

ELISA: 1:1000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

western blot: 1:500-1:1000 (Cell Lysate)

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

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General description

Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.

Specificity

This antibody recognizes FAK phosphorylated at Tyr397.

Immunogen

Epitope: Phosphorylated Tyr397
KLH-conjugated linear peptide corresponding to human FAK phosphorylated at Tyr397.

Application

Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay.
Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot peptide blocked on RAT2 cells transfected with Src.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blot in untransfected and Src-transfected RAT2 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-FAK (Tyr397) in 10 µg of untransfected and Src-transfected RAT2 cell lysates.

Target description

~125 kDa observed.
An uncharacterized band appears at ~190 kDa in some lysates.

Linkage

Replaces: 04-974

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
untransfected and Src-transfected RAT2 cell lysates

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jae Hoon Kim et al.
International journal of molecular sciences, 23(20) (2022-10-28)
PTK7 is a catalytically defective receptor protein tyrosine kinase upregulated in various cancers, including esophageal squamous cell carcinoma (ESCC). In previous studies, we observed a positive correlation between PTK7 expression levels and tumorigenicity in various ESCC cell lines and xenograft
Jennifer J Chia et al.
The Journal of clinical investigation, 126(11), 4331-4345 (2016-11-02)
Scleroderma is a group of skin-fibrosing diseases for which there are no effective treatments. A feature of the skin fibrosis typical of scleroderma is atrophy of the dermal white adipose tissue (DWAT). Adipose tissue contains adipose-derived mesenchymal stromal cells (ADSCs)
Won-Sik Shin et al.
Journal of cellular biochemistry, 118(9), 2887-2895 (2017-02-19)
Protein tyrosine kinase 6 (PTK6; also known as Brk) is closely related to the Src family kinases, but lacks a membrane-targeting myristoylation signal. Sublocalization of PTK6 at the plasma membrane enhances its oncogenic potential. To understand the mechanism(s) underlying the
Yashavantha L Vishweshwaraiah et al.
Nature communications, 12(1), 6615-6615 (2021-11-18)
Advances in protein design have brought us within reach of developing a nanoscale programming language, in which molecules serve as operands and their conformational states function as logic gates with precise input and output behaviors. Combining these nanoscale computing agents
Won-Sik Shin et al.
International journal of molecular sciences, 23(4) (2022-02-27)
Protein tyrosine kinase 7 (PTK7), a catalytically defective receptor protein tyrosine kinase, is upregulated in tumor tissues and cell lines of esophageal squamous cell carcinoma (ESCC). We showed that PTK7 plays an oncogenic role in various ESCC cell lines. However

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