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ABT273

Sigma-Aldrich

Anti-SUN1 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
SUN domain-containing protein 1, Protein unc-84 homolog A, Sad1/unc-84 protein-like 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SUN1(23353)

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This Item
MABT892HPA008346HPA008461
Anti-SUN1 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABT273

Anti-SUN1 Antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

12.10F, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, human

species reactivity

mouse, rat

species reactivity

human

species reactivity

human

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500

NCBI accession no.

NP_001124437

NCBI accession no.

NP_077771

NCBI accession no.

-

NCBI accession no.

-

General description

SUN domain-containing protein 1 (SUN1), also known as Protein unc-84 homolog A or Sad1/unc-84 protein like 1 (UNC84A), is a component of the SUN-protein-containing complexes also called LINC (linker of nucleoskeleton and cytoskeleton) complexes. These complexes link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis as well. Additionally, SUN1 it is important in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing. For instance, recent research demonstrates that the position of the nucleus in sensory epithelial cells, partly governed by SUN1, appears critical for normal hearing.

Immunogen

Epitope: Perinuclear space domain
MBP-tagged recombinant protein corresponding to the perinuclear space domain of human SUN1.

Application

Detect SUN1 using this Anti-SUN1 antibody validated for use in western blotting & ICC.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
Western Blotting Analysis: A 1:1;000 dilution from a representative lot detected SUN1 in 10 µg of HepG2, Jurkat, and PC3 cell lysate.
Western Blotting Analysis: A representative lot detected SUN1 recombinant protein (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498).
Immunocytochemistry Analysis: A representative lot detected SUN1 in human foreskin fibroblasts cells (Haque, F., et al. (2010). Journal of Biol. Chem. 285(5): 3487-3498).

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1;000 dilution of this antibody detected SUN1 in 10 µg of A431 cell lysate.

Target description

~ 110 kDa observed. The calculated molecular weight is 90 kDa, however Sun1 has been shown as a ~80-110 kDa band in western blots (Gob, E., et al. (2011). Commun. Integr Biol. 4(4):440-442). Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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