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APT165

Sigma-Aldrich

Caspase 3 Colorimetric Activity Assay Kit, DEVD

Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.

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Synonym(s):
CPP32
eCl@ss:
32161000

Quality Level

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable

NCBI accession no.

UniProt accession no.

detection method

colorimetric

shipped in

dry ice

Gene Information

human ... CASP3(836)

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Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

CaspaTag, Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

species reactivity (predicted by homology)

mammals

species reactivity (predicted by homology)

mammals

species reactivity (predicted by homology)

-

species reactivity (predicted by homology)

mammals

detection method

colorimetric

detection method

fluorometric

detection method

-

detection method

colorimetric

NCBI accession no.

NM_004346.3, NM_032991.2

NCBI accession no.

NM_004346.3, NM_032991.2

NCBI accession no.

-

NCBI accession no.

NM_001229.2, NM_032996.1

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.

Application

Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Research Category
Apoptosis & Cancer

Components

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90080): 1 mL of 3 mg/mL solution

Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

Storage and Stability

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Selda Gezginci-Oktayoglu et al.
Apoptosis : an international journal on programmed cell death, 17(1), 14-24 (2011-09-23)
Recent evidence suggested that tumor necrosis factor-alpha (TNF-α) and nerve growth factor (NGF) withdrawal activated a common apoptotic pathway. Here, we aimed to investigate the possible role of apoptotic Ras effectors RASSF1 and NORE1 in NGF reduction and TNF-α-related β
Pingjun Zhu et al.
Frontiers in cardiovascular medicine, 9, 856041-856041 (2022-04-12)
Mitophagy, known as the main mechanism of mitochondrial quality control, determines the pathophysiology of septic cardiomyopathy, although the precise regulatory mechanisms remain elusive. Data from the present study suggested that receptor-interacting protein kinase 3 (RIPK3) expression could be enhanced in
J M Eichhorn et al.
Cell death & disease, 4, e834-e834 (2013-10-05)
Microtubule inhibiting agents (MIAs) characteristically induce phosphorylation of the major anti-apoptotic Bcl-2 family members Mcl-1, Bcl-2 and Bcl-xL, and although this leads to Mcl-1 degradation, the role of Bcl-2/Bcl-xL phosphorylation in mitotic death has remained controversial. This is in part
Norma Alva et al.
Experimental physiology, 98(6), 1115-1124 (2013-01-29)
There is growing interest in using hypothermia to prevent hypoxic damage in clinical and experimental models, although the mechanisms regulated by hypothermia are still unclear. As reactive oxygen and nitrogen species are the main factors causing cellular damage, our objective
Alistair Taverner et al.
Journal of controlled release : official journal of the Controlled Release Society, 210, 189-197 (2015-05-20)
The intestinal epithelium functions to effectively restrict the causal uptake of luminal contents but has been demonstrated to transiently increase paracellular permeability properties to provide an additional entry route for dietary macromolecules. We have examined a method to emulate this

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