CBL1512F
Anti-Integrin αM [CD11b] Antibody, clone OX-42, FITC conjugated
clone OX-42, Chemicon®, from mouse
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CD11b
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biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody product type
primary antibodies
clone
OX-42, monoclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Itgam(25021)
Related Categories
Specificity
This antibody recognizes most macrophages (including resident peritoneal and activated macrophages), kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3.
FUSION PARTNER: NSO myeloma cell line.
FUSION PARTNER: NSO myeloma cell line.
Application
Flow cytometry
Immunohistochemical studies of rat fresh frozen tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15′ no post-fix).
Optimal working dilutions must be determined by the end user.
Immunohistochemical studies of rat fresh frozen tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15′ no post-fix).
Optimal working dilutions must be determined by the end user.
This Anti-Integrin αM CD11b Antibody, clone OX-42, FITC conjugated is validated for use in FC, IH for the detection of Integrin αM [CD11b].
Linkage
Replaces: MAB1404F
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
wgk_germany
WGK 2
Certificates of Analysis (COA)
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