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CBL208

Sigma-Aldrich

Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8

clone IT-Ks20.8, Chemicon®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

IT-Ks20.8, monoclonal

species reactivity

human

should not react with

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... KRT20(54474)
mouse ... Krt20(66809)
rat ... Krt20(286912)

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This Item
MABT845SAB5600092MAB3230
biological source

mouse

biological source

mouse

biological source

-

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

mouse

species reactivity

human

species reactivity

rat, canine, human, mouse

Gene Information

human ... KRT20(54474)
mouse ... Krt20(66809)
rat ... Krt20(286912)

Gene Information

human ... KRT20(54474)
mouse ... Krt20(66809)

Gene Information

human ... KRT20(54474)

Gene Information

human ... KRT10(3858)

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: 1:100-1:250, western blot: 1000-2000

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

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General description

IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.

Specificity

The antibody is specific for the 46 kDa polypeptide (cytokeratin 20).

Immunogen

Electrophoretically purified cytokeratin 20 from human intestinal mucosa

Application

Western blotting
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material

Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.

Optimal working dilutions must be determined by the end user.
Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).
Research Category
Cell Structure
Research Sub Category
Cytokeratins

Linkage

Replaces: MABT150

Physical form

Format: Purified
Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.

Storage and Stability

Once reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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P Harnden et al.
British journal of urology, 78(6), 870-875 (1996-12-01)
To investigate the dysregulation of cytokeratin 20 (CK20) expression in urothelial dysplasia and its potential as a diagnostic aid. Twenty-two patients were selected on the basis that they had undergone one or more biopsies showing dysplasia before the development of
The distribution and origin of keratin 20-containing taste buds in rat and human.
Zhang, C and Oakley, B
Differentiation, 61, 121-127 (1996)
Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins.
Demirkesen, C, et al.
Journal of cutaneous pathology, 22, 518-535 (1995)
I Moll et al.
Archives of dermatological research, 283(5), 300-309 (1991-01-01)
Human eccrine sweat gland ducts and benign and malignant eccrine poromas were studied for the expression of various cytokeratins (CK) and vimentin by applying immunoperoxidase and immunofluorescence microscopy to frozen or paraffin-embedded sections, and using two-dimensional gel electrophoresis and immunoblotting.
V J Möbus et al.
British journal of cancer, 69(3), 422-428 (1994-03-01)
Two human ovarian (OV-MZ-10, OV-MZ-15) and two colon cancer cell lines (CO-MZ-5, CO-MZ-6) were newly established in permanent cell culture. These cell lines have been maintained in vitro for 5-6 years, the passage number varying from 25 to 228. They

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