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Sigma-Aldrich

Bovine Collagen Type VI

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eCl@ss:
32160405

biological source

bovine

Quality Level

Assay

>90% (SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)

solubility

water: soluble at 20 °C

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

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This Item
C2124C4243CC054
assay

>90% (SDS-PAGE)

assay

-

assay

≥99.9% (SDS-PAGE)

assay

90% (Human collagen type III, SDS-PAGE)

form

liquid

form

liquid

form

liquid

form

liquid

concentration

0.5 mg/mL

concentration

6 mg/mL

concentration

2.9-3.2 mg/mL

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

water: soluble at 20 °C

solubility

-

solubility

-

solubility

water: soluble at 20 °C

General description

Product Source: Bovine placenta villi

Application

Coating material for cell culture studies

Formation of collagen gels

Type VI collagen standard

Antigen for antibody production

Physical form

Liquid in 500 mM acetic acid, no preservatives.

Preparation Note

Collagen is extracted by controlled and limited pepsin digestion, followed by selective salt precipitation.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze thaws. The collagen may be transferred into physiological buffers by dialysis at 2-8°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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