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Millipore

Direct Detect Spectrometer

By measuring amide bonds in protein chains, the Direct Detect Spectrometer system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding or redox potential.

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eCl@ss:
32160606
NACRES:
NB.22

Quality Level

manufacturer/tradename

Direct Detect®

technique(s)

protein quantification: suitable

General description

The Direct Detect infrared (IR)-based quantification system is an innovative combination of software-controlled instrumentation and EMD Millipore Corporation’s advanced membrane technology, optimized for the detection and quantification of proteins. Biomolecules are applied directly to a card-based hydrophilic polytetrafluoroethylene (PTFE) membrane that is transparent in most of the infrared spectral region. The Direct Detect system measures amide bonds in protein chains, accurately quantifying an intrinsic component of every protein without relying on amino acid composition, dye binding properties, or redox potential. Protein concentrations from 0.2 to 5 mg/mL can accurately be determined from a minimal sample volume (2 μL) without bio- or immuno-chemical staining. Sample analysis takes only minutes and in most cases can be performed directly from the buffered or native solution.

Components

1 Direct Detect Spectrometer

1 Instrument power adapter with 4 power cord/plug configurations (EU, UK, US, Japan)

1 Data cable (crossover cable for 10Base-T Ethernet standard, with RJ45 connectors)

1 Dell Netbook computer with Direct Detect software installed

1 Computer power adapter with world-wide plug adapter

1 Netbook stand

Direct Detect Assay-free cards (package of 50)

1 Direct Detect spotting tray

1 Desiccant pack replacement

1 Torx TX20 screw driver for replacing the IR source

1 Direct Detect Spectrometer Quick Start Card

Legal Information

DIRECT DETECT is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Learn how total protein quantitation with an infrared spectrometer may be a more accurate and universal alternative to Bradford, BCA, or UV-based methods.

Protocols

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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