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ECM220

Sigma-Aldrich

QCM Laminin Migration Assay (24-well, colorimetric)

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eCl@ss:
32161000

manufacturer/tradename

Chemicon®
QCM

Quality Level

technique(s)

cell based assay: suitable

detection method

colorimetric
fluorometric

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ECM506ECM210ECM200
manufacturer/tradename

Chemicon®, QCM

manufacturer/tradename

Chemicon®, QCM

manufacturer/tradename

Chemicon®, QCM

manufacturer/tradename

Chemicon®, QCM

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

cell based assay: suitable

technique(s)

activity assay: suitable, cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

General description

Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation.
Cell migration may be evaluated using several different methods; the most widely accepted being the Boyden Chamber assay. The Boyden Chamber system uses a two-chamber plate model in which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are stained and quantified. Most cells are sized from 30 to 50 μm can migrate through 3 to 10 μm pore.

Application

Research Category
Cell Structure
The Millipore QCM Laminin Migration Assay incorporates a 5 μm porous membrane Boyden chamber and mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin. The laminin is isolated from mouse sarcoma and it is enriched with mouse laminin 1 (laminin-111). This ECM promotes cell adhesion, proliferation as well as migration in many cell types such as embryonic stem cells, neural cells, liver cells and cancer cells. Cells interact with laminin through integrins α1β1, α2β2, α3β1, α6β1, α7β1, and α6β4. The assay is designed specifically to examine the migration of a subset of fibroblasts, cancer cells and neural cells.
Laminin protein is coated on the bottom of each chamber′s porous membrane as the driver for cell migration. A similarly BSA-coated control chamber(s) provides an appropriate migration control. Cells that migrate toward laminin are stained using crystal violet, a nucleic dye, extracted from the membrane surface, and quantified on a microplate reader using spectrophotometry.

Each kit provides sufficient materials for the evaluation of 24 samples.
This QCM Laminin Migration Assay incorporates a 5 um porous membrane, 24-well plate Boyden chamber & mouse laminin, a major extracellular matrix (ECM) protein in basement membrane, to examine cell migration dependent upon the presence of laminin.

Packaging

Sufficient for 24 samples

Components

ECM220-1
1. 24-well Cell Migration Plate Assembly - 5 μm: (Part No. 2005708) Two 24-well plates, each containing 12- 5 μm pore inserts per plate.
2. BSA 30% solution: (Part No. CS203352) One vial containing 0.5 mL of a 30% solution.
3. Cell Stain Solution*: (Part No. 90144) One bottle.
4. Extraction Buffer: (Part No. 90145) One bottle.
5. 24-well Stain Receiver Plate: (Part No. PIMWS2450) One each.
6. 96-Well Stain Quantitation Plate: (Part No. 2005870) One each.
7. Swabs: 50 ea
8. Forceps: 1 pair
*Caution: Cell Stain Solution contains a small amount of crystal violet, which is toxic if swallowed or inhaled, and may cause irritation to the eyes, respiratory system, and skin. Handle with caution.
ECM220-2
1. Laminin: (Part No. CS202685) Two vials each containing 60 μg in solution.

Storage and Stability

ECM220 should be used within four months from date of receipt. In that time:
  • ECM220-1 kit components should be stored at 2° to 8°C. DO NOT FREEZE.
  • ECM220-2 component must be stored at -20°C until needed.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

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Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

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WGK 3


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