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EZHS

Sigma-Aldrich

High Sensitivity Human Amyloid β40 and Amyloid β42 ELISA

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species reactivity

human

Quality Level

technique(s)

ELISA: suitable

assay range

inter-assay cv: <10
intra-assay cv: <10
standard curve range: 16 -500 pg/mL

detection method

colorimetric

storage temp.

2-8°C

Gene Information

human ... APP(351)

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This Item
EZHS42EZHS40MABN11
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, mouse

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

-

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

General description

Amyloid beta peptides have been implicated in the etiology of Alzheimer’s disease. Amyloid beta 40 is the most prominent peptide and Amyloid beta 42 is the neurotoxic form. The Amyloid beta 42/40-ratio (AB ratio) has been reported as a better indicator of the Alzheimer pathology. Millipore’s High Sensitivity Human Amyloid β40 and Amyloid β42 ELISA kits are used for the measurement of Amyloid β40 and Amyloid β42 ELISA in cerebrospinal fluid, cell culture supernatants, primary neurons and plasma in a 96-well format.

Specificity

The Amyloid β40 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-40, which causes a high selectivity for Aβ 40. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-42, Aβ1-43 and Aβ1-44.

The Amyloid β42 ELISA (HS) uses monoclonal anti-Aβ antibodies with high selectivity for human Aβ. The capture antibody recognizes the C-terminal end of Amyloid β1-42, which causes a high selectivity for Aβ42. The cross-reactivity of the used antibodies to other Amyloid peptides was tested by ELISA and BIACORE and shows no significant cross-reactivity to Aβ1-38, Aβ1-39, Aβ1-40, Aβ1-43 and Aβ1-44.

Used to detect/quantify: Amyloid β40 & Amyloid β42

Application

This High Sensitivity Human Amyloid β40 & Amyloid β42 ELISA is used to measure & quantify Amyloid β40 & Amyloid β42 levels in Neuroscience research.

These assays require 50 µl of sample and are overnight assays.

Research Category: Neuroscience

Research Sub-Category: Alzheimer′s Disease

Packaging

96-well plate

Storage and Stability

Components in the kit can be stored up to 2 weeks at 2-8°C

Other Notes

Contact technical support for information

Disclaimer

For research use only. Not for use in diagnostic procedures.

pictograms

CorrosionExclamation mark

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Warning

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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