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FCMAB103AP

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Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate

clone Ki-S5, Milli-Mark®, from mouse

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Synonym(s):
MKI67

KIA

Ki-67
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

Allophycocyanin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Ki-S5, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MKI67(4288)

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This Item
WH0004288M1P6834SAB5500134
biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

tissue culture supernatant

clone

Ki-S5, monoclonal

clone

7B8, monoclonal

clone

PP-67, monoclonal

clone

SP6, monoclonal

conjugate

Allophycocyanin conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... MKI67(4288)

Gene Information

human ... MKI67(4288)

Gene Information

human ... MKI67(4288)

Gene Information

human ... MKI67(4288)

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General description

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin′s lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.

Specificity

Antibody recognizes Ki67.

Immunogen

Nuclear protein preparation from the human cell line U937.50

Application

Detect Ki67 using this Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate validated for use in FC, IC, IH, FC, IH(P).
Research Category
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Flow Cytometry with Jurkat cells.

Target description

345 & 395 kDa observed

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to Allophycocyanin (APC) in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Analysis Note

Control
Stimulated Jurkat Cells

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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