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FCMAB288A6

Sigma-Aldrich

Milli-Mark® Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor647, clone Apu3

clone Apu3, Milli-Mark®, from rabbit

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eCl@ss:
32160702

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 647

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Apu3, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBB(7314)

Related Categories

General description

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Specificity

Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Immunogen

Immunogen used was di-ubiquitin linked at Lys63.

Application

This Milli-Mark Anti-Ubiquitin Antibody, Lys63-Specific-Alexa Fluor647, clone Apu3 is validated for use in FC for the detection of Ubiquitin.

Quality

Evaluated by flow cytometry using HeLa cells

Target description

Molecular weight varies.

Physical form

Purified recombinant rabbit monoclonal IgG1 conjugated to Alexa Fluor 647 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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