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GF307

Sigma-Aldrich

β-NGF Protein, Human Recombinant Animal Free

The Nerve Growth Factor is a well characterized neurotropic protein that plays a critical role in the development of sympathetic & some sensory neurons in the peripheral nervous system. Manufactured using all non-animal reagents.

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Synonym(s):
Nerve Growth Factor, NGF, BNGF, Beta NGF
eCl@ss:
32160405

Quality Level

assay

>98% (SDS-PAGE and HPLC)

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

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This Item
N5415GF301SRP3015
Nerve Growth Factor-ß human recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

N5415

Nerve Growth Factor-ß human

Sigma-Aldrich

SRP3015

Beta NGF human

UniProt accession no.

P01138

UniProt accession no.

P01138

UniProt accession no.

P23560

UniProt accession no.

P01138

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

impurities

≤1 EU/μg Endotoxin (β-NGF)

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

impurities

<0.1 EU/μg endotoxin, tested

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

-

NCBI accession no.

NM_002506.2

NCBI accession no.

-

NCBI accession no.

NM_001709.3, NM_170731.3, NM_170732.3, NM_170733.2, NM_170734.2, NM_170735.4

NCBI accession no.

-

General description

Nerve Growth Factor (β-NGF) is a well characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on nonneuronal tissues. β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity.
Product Source: E. coli

Specificity

Cross Reactivty
Chicken, Hamster, Mouse, Rat

Application

Research Category
Neuroscience
Research Sub Category
Growth Factors & Receptors

Quality

Bioactivity assay: Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human β-NGF is manufactured using all non-animal reagents

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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