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IM29L

Sigma-Aldrich

Anti-PAI-1 (Ab-1) Mouse mAb (2)

lyophilized, clone 2, Calbiochem®

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Synonym(s):
Anti-Plasminogen Activator Inhibitor-1

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

2, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... SERPINE1(5054)

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This Item
528215OP104LIM40
vibrant-m

IM29L

Anti-PAI-1 (Ab-1) Mouse mAb (2)

vibrant-m

528215

Anti-PAI-1 Mouse mAb (MA-33B8)

species reactivity

human

species reactivity

rabbit, human

species reactivity

human, mouse

species reactivity

human

Gene Information

human ... SERPINE1(5054)

Gene Information

human ... SERPINE1(5054)

Gene Information

human ... TP53(7157)

Gene Information

human ... MMP7(4316)

clone

2, monoclonal

clone

MA-33B8, monoclonal

clone

PAb1802, monoclonal

clone

141-7B2, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

isotype

IgG1

isotype

IgG

isotype

IgG1

isotype

IgG1

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General description

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.
Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.
This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).

Immunogen

Human
partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see application references)

Immunocytochemistry (see application references

Neutralization Studies (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Analysis Note

Positive Control
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.
Janicke, F., et al. 1994. Cancer Research.54, 2527.
Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.
Nagayama, M., et al. 1994. Cancer73, 1398.
Petersen, H., et al. 1994. Cancer Res.54, 120.
Keijer, J., et al. 1991. Blood78, 401.
Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.

Legal Information

Sold under license of U.S. Patent 5,422,245.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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