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MAB1618-AF488

Sigma-Aldrich

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor 488 Conjugate

clone 74.1, from mouse, ALEXA FLUOR 488

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Synonym(s):
Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1, Cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosoli
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

74.1, monoclonal

species reactivity

rat, Xenopus, fish, bovine, sheep, mouse, Drosophila, human

technique(s)

immunocytochemistry: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DYNC1I1(1780)

General description

Cytoplasmic dynein 1 intermediate chain 1 (UniProt Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) and Cytoplasmic dynein 1 intermediate chain 2 (UniProt Q0III3; also known as Cytoplasmic dynein intermediate chain 2, Dynein intermediate chain 2, cytosolic, DH IC-2) are encoded by the DYNC1I1 gene (Gene ID 613724) and DYNC1I2 gene (Gene ID 526329) in bovine, respectively. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∼1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 & IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.

Specificity

Reacts with all known forms of the 74 kDa intermediate chain (IC) subunit of mammaliam cytoplasmic dynein. The antigenic site has been mapped to the N-terminal 60 amino acids, a region conserved in intermediate chains encoded by both the DYNC1I1 and DYNC1I2 genes.

Application

Anti-Dynein Antibody, IC, cytosolic, clone 74.1, Alexa Fluor 488 Conjugate is an antibody against Dynein for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cytosolic dynein intermediate chains in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. MAB1618) is shown to be suitable also for Western blotting, immunocytochemistry, immunofluorescence, and immunoprecipitation applications.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cytosolic dynein intermediate chains in HeLa cells.

Target description

~74 kDa observed.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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