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MAB1951

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone P4G11

clone P4G11, Chemicon®, from mouse

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Synonym(s):
CD29
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P4G11, monoclonal

species reactivity

monkey, rabbit, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

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MAB1951FMAB1987MAB2000
species reactivity

monkey, rabbit, human

species reactivity

rabbit, monkey, human

species reactivity

human

species reactivity

human, pig

Gene Information

human ... ITGB1(3688)

Gene Information

human ... ITGB1(3688)

Gene Information

human ... ITGB1(3688)

Gene Information

human ... ITGB1(3688)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

culture supernatant

antibody form

purified immunoglobulin

clone

P4G11, monoclonal

clone

P4G11, monoclonal

clone

P4C10, monoclonal

clone

HB1.1, monoclonal

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

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Specificity

Reacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates.

Immunogen

Human IL-1beta activated endothelial cells

Application

Anti-Integrin β1 Antibody, clone P4G11 is an antibody against Integrin β1 for use in ELISA, FC, IC, IH, IH(P), IP, FUNC, CULT.
Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Physical form

Format: Purified
Protein A Purified immunoglobulin in 0.02M PBS, 0.25M NaCl, pH 7.6, with 0.1% NaN3.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Tonsil, human skin tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Anna M Joy et al.
Journal of cell science, 116(Pt 21), 4409-4417 (2003-09-18)
Glioma cells that migrate out of the main tumor mass into normal brain tissue contribute to the failure of most gliomas to respond to treatment. Treatments that target migratory glioma cells may enhance the therapeutic response. Multiple lines of evidence
Jesus Vega-Lugo et al.
The Journal of cell biology, 221(7) (2022-05-14)
Colocalization analysis of multicolor microscopy images is a cornerstone approach in cell biology. It provides information on the localization of molecules within subcellular compartments and allows the interrogation of known molecular interactions in their cellular context. However, almost all colocalization
Convergence of eicosanoid and integrin biology: 12-lipoxygenase seeks a partner.
Tang, K; Cai, Y; Joshi, S; Tovar, E; Tucker, SC; Maddipati, KR; Crissman, JD; Repaskey, WT; Honn, KV
Molecular Cancer null
C Radel et al.
American journal of physiology. Heart and circulatory physiology, 288(2), H936-H945 (2004-10-09)
To identify the role of caveolin-1 in integrin mechanotransduction, we exposed bovine aortic endothelial cells to 10 dyn/cm2 of laminar shear stress. Caveolin-1 was acutely and transiently phosphorylated with shear, occurring downstream of beta1-integrin activation as the beta1-integrin blocking antibody
Francesca Ricciardiello et al.
Cell death & disease, 9(3), 377-377 (2018-03-09)
Cancer aberrant N- and O-linked protein glycosylation, frequently resulting from an augmented flux through the Hexosamine Biosynthetic Pathway (HBP), play different roles in tumor progression. However, the low specificity and toxicity of the existing HBP inhibitors prevented their use for

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