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MAB2610

Sigma-Aldrich

Anti-Zyxin Antibody, clone 14E11.1

clone 14E11.1, from mouse

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Synonym(s):
Zyxin2, Zyxin, Zyxin-2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

14E11.1, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Zyx(22793)

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This Item
Z4751Z0377SAB1404556
antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

14E11.1, monoclonal

clone

polyclonal

clone

ZOL301, monoclonal

clone

2D1, monoclonal

species reactivity

human, mouse, rat

species reactivity

mouse, human, rat

species reactivity

mouse, rat, human

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

indirect immunofluorescence: 1:100 using Rat1 cells, western blot (chemiluminescent): 1:1,000 using whole extract of human HeLa cells, western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 cells

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of HeLa cells.

technique(s)

capture ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

isotype

IgMκ

isotype

-

isotype

IgG2a

isotype

IgG2aκ

General description

Zyxin, is a cytosolic, focal adhesion protein belonging to the Ajuba family and containing 3 LIM zinc-binding domains. It associates with the actin cytoskeleton near the adhesion plaques and, during mitosis, with microtubules. Zyxin localizes to stress fibers, cell-cell junctions and colocalizes with vinculin, paxillin and VASP at focal adhesions but not at the tips of lamellipodia. Zyxin is involved in the regulation of actin dynamics and is a likely component of the signal transduction pathway mediating adhesion-stimulated changes in gene expression. Zyxin displays an actin nucleating activity that is dependent on its amino terminal proline-rich motifs and independent of Arp2/3 complex. Zyxin appears to function as an adaptor or docking protein to organize protein complexes important for regulated actin cytoskeleton dynamics.

Immunogen

KLH-conjugated linear peptide corresponding to human Zyxin.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Zyxin in NIH/3T3, A431, and HeLa cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-Zyxin Antibody, clone 14E11.1 is validated for use in WB, IC for the detection of Zyxin.

Quality

Evaluated by Western Blot in HUVEC cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Zyxin in 10 µg of HUVEC cell lysate.

Target description

~84 kDa observed. A non-specific band may be observed at ~36 kDa
The calculated molecular weight is 61 kDa Zyxin is a phosphoprotein with an apparent molecular weight of 82–84 kDa (van der Gaag, E., et al. (2002). Journal of Investigative Dermatology. 118:246–254.). As a substrate of caspases, Zyxin can be cleaved as 30, 37, 48 and 70 kDa (C-B Chan, C-B., et al. (2007). Cell Death and Differentiation. 14:1688–1699.)

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HUVEC cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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