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Key Documents

MAB308

Sigma-Aldrich

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2

ascites fluid, clone 4F10.2, Chemicon®

Synonym(s):

DBH

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4F10.2, monoclonal

species reactivity

rat, bovine, sheep

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Dbh(280758)
rat ... Dbh(25699)

General description

Dopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.

Specificity

Dopamine beta hydroxylase (DBH).

Immunogen

Purified bovine DBH.

Application

Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.
Indirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.

Optimal working dilutions must be determined by the end user.

Immunohistochemistry(paraffin):
Representative images from a previous lot.

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

Quality

Immunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).

Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord

Linkage

Replaces: MAB394-250UG; MAB394-100UG; MAB394-25UG

Physical form

Ascites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential activation of chemically identified neurons in the caudal nucleus of the solitary tract in non-entrained rats after intake of satiating vs. non-satiating meals.
Kreisler, AD; Davis, EA; Rinaman, L
Physiology & Behavior null
The rate of fall of blood glucose determines the necessity of forebrain-projecting catecholaminergic neurons for male rat sympathoadrenal responses.
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Glucocorticoid enhancement of memory requires arousal-induced noradrenergic activation in the basolateral amygdala.
Roozendaal, B; Okuda, S; Van der Zee, EA; McGaugh, JL
Proceedings of the National Academy of Sciences of the USA null
Michael D Schwartz et al.
eNeuro, 3(2) (2016-03-30)
Hypocretin 1 and 2 (Hcrts; also known as orexin A and B), excitatory neuropeptides synthesized in cells located in the tuberal hypothalamus, play a central role in the control of arousal. Hcrt inputs to the locus coeruleus norepinephrine (LC NE)
Hermina Nedelescu et al.
Brain structure & function, 222(1), 317-339 (2016-04-09)
The vermis or "spinocerebellum" receives input from the spinal cord and motor cortex for controlling balance and locomotion, while the longitudinal hemisphere region or "cerebro-cerebellum" is interconnected with non-motor cortical regions, including the prefrontal cortex that underlies decision-making. Noradrenaline release

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