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MAB3140

Sigma-Aldrich

Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9

clone 4E9, Chemicon®, from mouse

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Synonym(s):
NHE1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4E9, monoclonal

species reactivity

amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)

species reactivity (predicted by homology)

mouse, mammals, rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

isotype

IgG1

suitability

not suitable for immunoprecipitation

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC9A1(6548)

General description

Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.

Specificity

Na+/H+ exchanger, isoform NHE1

Immunogen

Epitope: C-terminus
MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.

Application

Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.

Immunoblotting:
1:1,000 dilution used on a previous lot.

Optimal working dilutions must be determined by the user.

Quality

Routinely evaluated by Western Blot on Human Kidney lysates.

Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.

Target description

~100-110 kDa

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Kidney tissue (basolateral membrane)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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