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MAB328

Sigma-Aldrich

Anti-Oligodendrocytes Antibody, clone CE-1

ascites fluid, clone CE-1, Chemicon®

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Synonym(s):
MOSP
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CE-1, monoclonal

species reactivity

rat, mouse, chicken, monkey, feline, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotype

IgM

suitability

not suitable for Western blot

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... OLIG2(10215)

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MAB1580SAB1404798I9018
clone

CE-1, monoclonal

clone

NS-1, monoclonal

clone

3C9, monoclonal

clone

SY5, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... OLIG2(10215)

Gene Information

human ... OLIG2(10215)

Gene Information

human ... OLIG2(10215)

Gene Information

human ... IVL(3713)

species reactivity

rat, mouse, chicken, monkey, feline, human

species reactivity

human, hamster, rat, mouse

species reactivity

human

species reactivity

canine, gorilla, owl monkey, pig, human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

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Specificity

In neonatal rat glia MAB328 stains oligodendrocytes and does not react with type 1 astrocytes, type 2 astrocytes or fibroblasts. Does not stain cultures of rat Schwann cells. In tissue sections MAB328 labels oligodendrocytes and CNS myelin with no labeling of peripheral myelin. MAB328 detects MOSP which is expressed at day 4-5 of neonatal rat, which is about 1-2 after the appearance of GC or sulfatide. The initial expression of MOSP occurs at the stage in development when oligodendrocytes are elaborating processes and just beginning to form membrane sheets (Mu QQ & C Dyer, 1994).

Immunogen

Rat glial membranes and whole brain white matter.

Application

Immunocytochemistry

Immunohistochemistry at 1:1,000-1:5000

Not suggested for use in Western blot.

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
This Anti-Oligodendrocytes Antibody, clone CE-1 is validated for use in IP, IC, IH, IH(P) for the detection of Oligodendrocytes.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ying Ding et al.
BMC neuroscience, 10, 35-35 (2009-04-21)
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The poor regeneration capability of the mammalian hearing organ has initiated different approaches to enhance its functionality after injury. To evaluate a potential neuronal repair paradigm in the inner ear and cochlear nerve we have previously used embryonic neuronal tissue
Adela Quesada et al.
Journal of neuroscience research, 89(5), 674-688 (2011-02-22)
The retina of nonmammalian vertebrates has a loose myelin that enwraps the large axons of the ganglion cells in all areas, whereas that of mammals lacks myelin, with some exceptions, such as the rabbit retina, which shows compact myelin restricted
Nicole M Jones et al.
Journal of neurochemistry, 89(1), 157-167 (2004-03-20)
Hypoxic preconditioning (HP) 24 h before hypoxic-ischemic (HI) injury confers significant neuroprotection in neonatal rat brain. Recent studies have shown that the mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase (PI3K) intracellular signaling pathways play a role in the induction of tolerance
Dheeraj Pelluru et al.
The European journal of neuroscience, 43(10), 1298-1306 (2015-09-16)
A distributed network of neurons regulates wake, non-rapid eye movement (NREM) sleep, and REM sleep. However, there are also glia in the brain, and there is growing evidence that neurons and astroglia communicate intimately to regulate behaviour. To identify the

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