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MAB3448

Sigma-Aldrich

Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9

ascites fluid, clone 1MOD-1A9, Chemicon®

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Synonym(s):
HP1b
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1MOD-1A9, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CBX1(10951)

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This Item
MABE664SAB1402535MAB3584
antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

clone

1MOD-1A9, monoclonal

clone

5E9.1, monoclonal

clone

4E12, monoclonal

clone

2HP-1H5, monoclonal

species reactivity

mouse, human

species reactivity

human

species reactivity

human

species reactivity

mouse, human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

western blot: suitable

technique(s)

immunofluorescence: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

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General description

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Specificity

Reacts with Heterochromatin protein-1 b (HP1b).

Immunogen

Recombinant mouse HP1beta.

Application

Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
Western blot: 1:500-1:5,000


Immunofluorescence: 1:500-1:5,000


Immunohistochemistry: 1:500-1:5,000


Immunoprecipitation: 1:500 - 1:5,000


ELISA: 1:500-1:5,000


Optimal working dilutions must be determined by the end user.

Target description

28 kDa

Analysis Note

Control
HeLa, 293 or NIH/3T3 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Sarah Herberg et al.
Scientific reports, 5, 14236-14236 (2015-09-22)
Transposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal development. Although their
Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
Testing null
Kyoko Hiragami-Hamada et al.
Nature communications, 7, 11310-11310 (2016-04-20)
Histone H3 trimethylation of lysine 9 (H3K9me3) and proteins of the heterochromatin protein 1 (HP1) family are hallmarks of heterochromatin, a state of compacted DNA essential for genome stability and long-term transcriptional silencing. The mechanisms by which H3K9me3 and HP1
Yong-Nan Xu et al.
Journal of assisted reproduction and genetics, 28(4), 325-333 (2010-11-26)
To evaluate DNA synthesis and epigenetic modification in mouse oocytes during the first cell cycle following the injection of human or hamster sperm. Mouse oocytes following the injection of human and hamster sperm and cultured in M16 medium. Male and
Erik Müllers et al.
Aging cell, 16(3), 575-584 (2017-03-28)
In response to DNA damage, a cell can be forced to permanently exit the cell cycle and become senescent. Senescence provides an early barrier against tumor development by preventing proliferation of cells with damaged DNA. By studying single cells, we

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