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MAB388

Sigma-Aldrich

Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1

clone PLPC1, Chemicon®, from mouse

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Synonym(s):
PLP
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PLPC1, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PLP1(5354)

Specificity

Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.

Immunogen

Epitope: C-terminus
Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.

Application

Detect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins
Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.

Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.

Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.

Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.

Optimal working dilutions must be determined by end user.

Target description

23-25 kDa

Linkage

Replaces: MAB388

Physical form

Format: Purified
Protein A purified
Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Aline M Thomas et al.
NeuroImage, 204, 116245-116245 (2019-10-13)
Multiple sclerosis (MS) is an autoimmune disorder that targets myelin proteins and results in extensive damage in the central nervous system in the form of focal lesions as well as diffuse molecular changes. Lesions are currently detected using T1-weighted, T2-weighted
Sachiko Homma et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(30), 7974-7986 (2007-07-27)
The heat shock transcription factors (Hsfs) are responsible for the heat shock response, an evolutionarily conserved process for clearance of damaged and aggregated proteins. In organisms such as Caenorhabditis elegans, which contain a single Hsf, reduction in the level of
Tapas Makar et al.
Stem cell research & therapy, 9(1), 127-127 (2018-05-04)
Adult bone marrow contains stem cells that replenish the myeloid and lymphoid lineages. A subset of human and mouse CD34+ bone marrow stem cells can be propagated in culture to autonomously express embryonic stem cell genes and embryonic germ layer
Raquel Bello-Morales et al.
PloS one, 6(5), e19388-e19388 (2011-05-17)
The velocity of the nerve impulse conduction of vertebrates relies on the myelin sheath, an electrically insulating layer that surrounds axons in both the central and peripheral nervous systems, enabling saltatory conduction of the action potential. Oligodendrocytes are the myelin-producing
Tuancheng Feng et al.
Brain : a journal of neurology, 143(7), 2255-2271 (2020-06-24)
TMEM106B encodes a lysosomal membrane protein and was initially identified as a risk factor for frontotemporal lobar degeneration. Recently, a dominant D252N mutation in TMEM106B was shown to cause hypomyelinating leukodystrophy. However, how TMEM106B regulates myelination is still unclear. Here

Articles

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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