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MAB4334Z

Sigma-Aldrich

Anti-MDR1 Antibody, clone UIC2, azide free

clone UIC2, Chemicon®, from mouse

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Synonym(s):
P-glycoprotein, CD243, p170, Pgp, MAB4334
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

UIC2, monoclonal

species reactivity

primate, human

should not react with

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... ABCB1(5243)

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MAB4334MAB4163MAB4120
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

culture supernatant

clone

UIC2, monoclonal

clone

UIC2, monoclonal

clone

MC57, monoclonal

clone

JSB-1, monoclonal

species reactivity

primate, human

species reactivity

primate, human

species reactivity

human

species reactivity

human, hamster

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

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General description

UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE′s UIC2 Shift assay - see product ECM905).

Specificity

P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)

Immunogen

Epitope: conformational extracellular epitope, azide free
Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.

Application

Detect MDR1 using this Anti-MDR1 Antibody, clone UIC2, azide free validated for use in FC, IF, IP, IH.
Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems

Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 ug per 1 μl of cell lysate

Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors

Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors IHC on frozen and paraffin sections: 1 - 10 μg/mL
Research Category
Metabolism
Research Sub Category
Toxicology & Drug Resistance

Physical form

Format: Purified
Purified immunoglobulin from Protein A Sepharose chromatography. Sterile filtered in 0.01 M PBS, no preservative.

Storage and Stability

Maintain at 2-8°C for up 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Eugene Mechetner
Methods in molecular biology (Clifton, N.J.), 378, 175-193 (2008-07-09)
Acquired and intrinsic multidrug resistance is the major reason for the failure of anticancer chemotherapy. The most important component of clinical multidrug resistance is mediated by P-glycoprotein (Pgp), an ABC transporter encoded by the MDR1 gene and expressed on the

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