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MABC1032

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Anti-Pro-Atrial Natriuretic Peptide Antibody, clone 11E3.9

clone 11E3.9, from mouse

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Synonym(s):
CDD-ANF, Pro-Atrial Natriuretic Peptide, Natriuretic peptides A, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, Prepronatriodilatin
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11E3.9, monoclonal

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NPPA(4878)

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This Item
AB2232CBL66MABX7132
species reactivity

rat, human

species reactivity

rat, mouse, human

species reactivity

human

species reactivity

human

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

clone

11E3.9, monoclonal

clone

polyclonal

clone

A6, monoclonal

clone

13G12, monoclonal

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), radioimmunoassay: suitable

technique(s)

ELISA: suitable, western blot: suitable

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General description

Natriuretic peptides A (UniProt P01160; also known as Atriopeptin, Cardiodilatin, Cardiodilatin-related peptide, Cardionatrin, CDD, CDD-ANF, CDP, Natriuretic peptide precursor A, Prepronatriodilatin) is encoded by the NPPA (also known as ANF, ANP, ATFB6, ATRST2, PND) gene (Gene ID 4878) in human. Atrial natriuretic peptide (ANP), also called atrial natriuretic factor, is a cardiac hormone that reduces high blood pressure by promoting salt excretion, decreasing blood volume, and relaxing vessel tension in a receptor-dependent manner. Deficiency in either ANP or its receptor causes spontaneous hypertension in knockout mice. ANP is initially synthesized in cardiac myocytes as a 153 a.a. precursor with a signal (a.a.1-25) and a propeptide (a.a. 26-123) sequence. The removal of the signal peptide yields pro-ANP (a.a. 26-151), which is further cleaved by the transmembrane cardiac serine protease Corin to yield mature ANP (a.a. 124-151), also known as atrial natriuretic factor (ANF). ANP is a short-lived molecule partly due to its proteolytic degradation by the kidney Neprilysin, also known as neutral endopeptidase 24.11.

Specificity

Expected to react with both prepro- and pro-atrial natriuretic peptide (ANP), but not mature ANP (a.a. 124-151 of prepro sequence).

Immunogen

Epitope: Propeptide (a.a. 26-123)
His-tagged recombinant protein corresponding to human Pro-Atrial Natriuretic Peptide.

Application

Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Pro-Atrial Natriuretic Peptide Antibody, clone 11E3.9 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Pro-Atrial Natriuretic Peptide.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected human Pro-Atrial Natriuretic Peptide in 10 µg of human heart tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected human Pro-Atrial Natriuretic Peptide in rat cardiac myocytes.

Quality

Evaluated by Western Blotting in rat heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected human Pro-Atrial Natriuretic Peptide protein in 10 µg of rat heart tissue lysate.

Target description

~17 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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