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MABC1109

Sigma-Aldrich

Anti-Mucin-9 Antibody, clone 7E10

clone 7E10, from mouse

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Synonym(s):
Oviduct-specific glycoprotein, Estrogen-dependent oviduct protein, Oviductal glycoprotein, Oviductin
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7E10, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... OVGP1(5016)

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This Item
MABC1608MABC1608MMABT899
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

7E10, monoclonal

clone

5E11, monoclonal

clone

monoclonal

clone

MDA-3E1, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human,

species reactivity

human, mouse

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

packaging

-

packaging

antibody small pack of 25 μL

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), proximity ligation assay: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), proximity ligation assay: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

General description

Oviduct-specific glycoprotein (UniProt: Q12889; also known as Estrogen-dependent oviduct protein, Mucin-9, Muc-9, Oviductal glycoprotein, Oviductin) is encoded by the OVGP1 (also known as MUC9, OGP) gene (Gene ID: 5016) in human. The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats. Mucin-9 belongs to the glycosyl hydrolase 18 family that plays a key role in sperm capacitation, fertilization, and development of early embryos. It is secreted from non-ciliated oviductal epithelial cells and its expression is highest at the time of ovulation. Mucin-9 is synthesized with a 21 amino acid signal peptide that is subsequently cleaved off to generate mature protein. Mucin-9 is an estrogen-dependent protein that is secreted into the lumen of the oviduct and associates with the zona pellucida of postovulatory oocytes in oviductal transit, but is absent in ovarian oocytes.

Specificity

Clone 7E10 detects Mucin-9 (Oviductin) in human oviduct sections.

Immunogen

A mixture of GST-tagged recombinant fragments corresponding to 81 amino acids from the N-terminal half and 112 amino acids from the C-terminal region of human Mucin-9/Oviductin.

Application

Anti-Mucin-9, clone 7E10, Cat. No. MABC1109, is mouse monoclonal antibody that detects Mucin-9 (Oviductin) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Mucin-9 in human placenta tissue.

Western Blotting Analysis: A representative lot detected Mucin-9 in Western Blotting applications (Maines-Bandiera, S., et. al. (2010). Int J Gynecol Cancer. 20(1):16-22).

ELISA Analysis: A representative lot detected Mucin-9 in ELISA applications (Maines-Bandiera, S., et. al. (2010). Int J Gynecol Cancer. 20(1):16-22).

Immunohistochemistry Analysis: A representative lot detected Mucin-9 in Immunohistochemistry applications (Maines-Bandiera, S., et. al. (2010). Int J Gynecol Cancer. 20(1):16-22).
Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunohistochemistry in human oviduct tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Mucin-9 in human oviduct tissue.

Target description

75.42 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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