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MABC1793

Sigma-Aldrich

Anti-RNF7 Antibody, clone SAG-10

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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

SAG-10, monoclonal

mol wt

calculated mol wt 12.68 kDa
observed mol wt ~12 kDa

purified by

using protein G

species reactivity

human, mouse

packaging

antibody small pack of 100 μg

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

mouse ... RNF7(9616)

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MABC1793

Anti-RNF7 Antibody, clone SAG-10

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Anti-AUF1 Antibody, clone 5B9

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Anti-REST Antibody, clone 12C11

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Anti-CD25 Antibody, clone 7D4

species reactivity

human, mouse

species reactivity

human

species reactivity

chicken, human, mouse

species reactivity

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

Gene Information

mouse ... RNF7(9616)

Gene Information

human ... HNRNPD(3184)

Gene Information

chicken ... REST(422623)

Gene Information

-

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rat

clone

SAG-10, monoclonal

clone

5B9, monoclonal

clone

12C11, monoclonal

clone

7D4, monoclonal

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General description

RING-box protein 2 (UniProt: Q9UBF6; also known as Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein) is encoded by the RNF7 (also known as RBX2, ROC2, SAG) gene (Gene ID: 9616) in human. RNF7/RBX2 and RBX1 are members of RING components of Cullin-RING ligases that are required for their enzymatic activity. RBX1 is shown to be constitutively expressed and binds to CUL 1-4; however, RNF7/RBX2 is induced by stress and associates with CUL5 and CUL1. RNF7/RBX2 is expressed in heart, liver, skeletal muscle, and pancreas and is present in cytoplasm and in the nucleus. RNF7/RBX2 is reported to negatively modulate β-TrCP1 levels by shortening its protein half-life by promoting its ubiquitylation via atypical K11-linkage. It can serve as an antioxidant when acting alone or as an E3 ligase when complexed with other components of E3 ubiquitin ligases. It also acts as a survival protein by inhibiting apoptosis induced by a variety of stresses. Overexpression of RNF7/RBX2 is shown to inhibit apoptosis induced by redox, nitric oxide, ischemia, heat shock, and neurotoxins, both in vivo and in vitro. Overexpression of RNF7/RBX2 is observed in multiple human primary tumor tissues and its silencing by siRNA does not affect normal cell growth, but inhibits growth of cancer cells, both in vitro and in vivo by induction of apoptosis. Its silencing is also reported to sensitize radioresistant H1299 and U87 cells to ionizing radiation. (Ref.: Kaung, P., et al. (2016). Sci. Rep. 6; 37441; Jia, L., et al. (2010). Clin Cancer Res; 16(3); 814-824; Tan, M., et al. (2010), Free Rad. Biol. Med. 49(6); 976-983).

Specificity

Clone SAG-10 is a mouse monoclonal antibody that specifically detects Ring finger protein 7 (RNF7).

Immunogen

GST-tagged recombinant fragment corresponding to 70 amino acids from human Ring finger protein 7 (RNF7).

Application

Quality Control Testing

Evaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected RNF7 in PANC-1 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:125 dilution from a representative lot detected RNF7 in H1299 cell lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated RNF7 in Immunoprecipitation application (Kuang, P. et al. (2016). Sci Rep. 6:37441).

Immunohistochemistry Applications: A representative lot detected RNF7 in Immunohistochemistry applications (Jia, L., et al. (2010). Clin Cancer Res.16(3):814-24).

Western Blotting Analysis: A representative lot detected RNF7 in Western Blotting applications (Jia, L., et al. (2010). Clin Cancer Res. 6(3):814-24; Tan, M., et al. (2010). Free Radic Biol Med. 49(6):976-83; Kuang, P. et al. (2016). Sci Rep. 6:37441).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-RNF7, clone SAG-10, Cat. No. MABC1793, is a mouse monoclonal antibody that detects RNF7 and is tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


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