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MABC567

Sigma-Aldrich

Anti-Somatoliberin/GHRH Antibody, clone 1B4.2

clone 1B4.2, from mouse

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Synonym(s):
Somatoliberin, Growth hormone-releasing factor, GRF, Growth hormone-releasing hormone, GHRH, Somatocrinin, Somatorelin, Sermorelin, Somatoliberin/GHRH
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B4.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

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This Item
MABC531SAB1405868SAB1300905
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

1B4.2, monoclonal

clone

1H1.1, monoclonal

clone

polyclonal

clone

polyclonal

isotype

IgG1κ

isotype

IgG1κ

isotype

-

isotype

-

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

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General description

The human GHRH (also known as GHRF) gene (Gene ID 2691) encodes the 108 aa Somatoliberin preproprotein (UniProt P01286; also known as GHRH, GRF, Growth hormone-releasing factor, Growth hormone-releasing hormone, INN: Sermorelin, Sermorelin, Somatocrinin, Somatorelin). Post-translatioinal removal of the signal peptide (aa 1-20) and the two propeptide sequences (aa 21-31 and aa 76-108) releases the 44 aa mature Somatoliberin (aa 32-75) that functions as a ligand to stimulate growth hormone (GH) release from the pituitary. Variant GHRH receptors are found in several types of tumors. Antagonists against such receptors are reported to inhibit tumors growth. Defects in GHRH gene cause dwarfism and hypersecretion of somatoliberin is linked to gigantism.

Specificity

Clone 1B4.2 is expected to detect prepro-, pro-, and mature somatoliberin.

Immunogen

Epitope: Mature somatoliberin
KLH-conjugated linear peptide corresponding to mature human somatoliberin sequence.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Somatoliberin/GHRH in human cerebral cortex tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-Somatoliberin Antibody, clone 1B4.2 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of GHRH .

Quality

Evaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Somatoliberin/GHRH in 10 µg of human lung tissue lysate.

Target description

~12 kDa observed. Preprosomatoliberin (12.4 kDa calculated); Prosomatoliberin (10.9 kDa calculated); Mature somatoliberin (10.4 kDa calculated). Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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