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MABE1790

Sigma-Aldrich

Anti-ADAR1 Antibody, clone RD4B11

clone RD4B11, from rat

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Synonym(s):
Double-stranded RNA-specific adenosine deaminase, EC: 3.5.4.37, DRADA, RNA adenosine deaminase 1
eCl@ss:
32160702

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RD4B11, monoclonal

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

mouse ... Adar(56417)

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Anti-ADAR2 Antibody, clone 1.3.1 clone 1.3.1, from mouse

MABE889

Anti-ADAR2 Antibody, clone 1.3.1

clone

RD4B11, monoclonal

clone

1.3.1, monoclonal

clone

polyclonal

clone

ADAR2-8, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

Gene Information

mouse ... Adar(56417)

Gene Information

human ... ADARB1(104)

Gene Information

human ... ADAR(103)

Gene Information

human ... ADARB1(104)

species reactivity

mouse, human

species reactivity

human

species reactivity

human

species reactivity

human

isotype

IgG2aκ

isotype

IgG1κ

isotype

-

isotype

-

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General description

Double-stranded RNA-specific adenosine deaminase (UniProt: Q99MU3; also known as EC: 3.5.4.37, DRADA, RNA adenosine deaminase 1) is encoded by the Adar gene (Gene ID: 56417) in murine species. ADAR catalyzes the editing of adenosine-to-inosine (A to I) by deaminating genomically encoded A-to-I in double stranded RNA (DsRNA). A-to-I editing predominantly occurs in noncoding, repetitive elements and short interspersed nuclear elements (SINEs). A-to-I editing affects gene expression and function in several ways, including mRNA translation by changing codons and hence the amino acid sequence of proteins. It also affects pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; and genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication. In mammals three different ADAR proteins have been described: ADAR1, -2, and -3. ADAR1 is shown to be expressed during embryonic and postnatal development and is present predominantly in the nucleus. Highest levels of ADAR1 are reported in brain and spleen and lowest levels are observed in liver. Five different isoforms of ADAR1 have been reported that are produced by alternative splicing. Isoforms 1 and 2 (long forms) are predominantly found in cytoplasm and shuttle between the cytoplasm and nucleus. Isoforms 3 and 4 (short forms starting at Met-519) are exclusively found in the nucleolus. Under normal conditions long forms are dominant, however, under conditions of inflammation short forms are selectively induced. (Ref.: Liddicoat, BJ et al., (2015). Science 349 (6252); 1115-1120).

Specificity

Clone RD4B11 is a rat monoclonal antibody that detects Double-stranded RNA-specific adenosine deaminase in human and murine cells.

Immunogen

His-tagged recombinant fragment corresponding to 401 amino acids from the internal region of murine Double-stranded RNA-specific adenosine deaminase (ADAR1). Immunogen sequnece is conserved isoforms 1 and 2.

Application

Anti-ADAR1, clone RD4B11, Cat. No. MABE1790, is a highly specific rat monoclonal antibody that targets ADAR1 and has been tested for use in Western Blotting.
Western Blotting Analysis: A representative lot detected ADAR1 in Western Blotting applications (Liddicoat, B.J., et. al. (2015). Science. 349(6252):1115-20).

Quality

Evaluated by Western Blotting in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.

Western Blotting Analysis: 4 µg/mL of this antibody detected ADAR1 in Kusa4b10 mouse cell line overexpressing mouse ADAR1 p110.

Target description

~110 kDa observed; 130.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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