MABE241
Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12
ascites fluid, clone 51TA-2H1, from mouse
Synonym(s):
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
About This Item
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biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
51TA-2H1, monoclonal
species reactivity
human
technique(s)
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10/pSer28), dimethylation (Lys9/Lys27)
Gene Information
human ... HIST1H3F(8968)
General description
Specificity
Immunogen
Application
Epigenetics & Nuclear Function
Histones
Dot Blot Analysis: A representative lot was used by an independent laboratory in unmodified and modified Histone H3 peptides. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739ā1754.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in NIH/3T3 cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739ā1754.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse testis tissue. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739ā1754.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739ā1754.)
Quality
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Histone H3 on 10 Āµg of untreated and etoposide acid extract treated HeLa cell lysates.
Target description
Physical form
Storage and Stability
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20Ā°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Untreated and etoposide acid extract treated HeLa cell lysates.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a productās label following the words āLotā or āBatchā.
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