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MABF151D

Sigma-Aldrich

Anti-mouse IFN gamma, FITC, clone XMG1.2 Antibody

clone XMG1.2, from rat, FITC conjugate

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Synonym(s):
Interferon gamma (IFN-gamma), IFN gamma
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

XMG1.2, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

Gene Information

mouse ... Ifng(15978)

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conjugate

FITC conjugate

conjugate

Allophycocyanin conjugate

conjugate

PE-Cy7

conjugate

PE-Cy7

Gene Information

mouse ... Ifng(15978)

Gene Information

mouse ... Ifng(15978)

Gene Information

mouse ... Ifng(15978)

Gene Information

mouse ... Ifng(15978)

biological source

rat

biological source

rat

biological source

rat

biological source

rat

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

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General description

The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells, which allow the cytokine to modulate innate immunity, when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein, e.g. by flow cytometry, as well as for quantitative analysis of the secreted protein by ELISA, when paired with an appropriate secondary antibody. This clone is also widely used for neutralization of the functional activity of IFN-g in a variety of assays.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-mouse IFN gamma Antibody, FITC, clone XMG1.2, Cat. No. MABF1517 is validated for use in Flow Cytometry, for the detection of IFN gamma.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected IFN gamma in one million C57Bl/6 splenocytes.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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