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MABF2774

Sigma-Aldrich

Anti-Dengue Virus 2 NS1 Antibody

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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

164, monoclonal

mol wt

calculated mol wt 48 kDa

species reactivity

virus

packaging

antibody small pack of 100 μg

technique(s)

ELISA: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

dry ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

Unknown ... POLY(1494449)

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MABF2774

Anti-Dengue Virus 2 NS1 Antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

164, monoclonal

clone

2H6, monoclonal

clone

1H10-6, monoclonal

clone

D3-2H2-9-21, monoclonal

Gene Information

Unknown ... POLY(1494449)

Gene Information

vaccinia virus ... NS1(956533)

Gene Information

-

Gene Information

-

species reactivity

virus

species reactivity

influenza A virus

species reactivity

human

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

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General description

Genome polyprotein (UniProt: P29990) following its synthesis is cleaved into 13 different proteins in Dengue virus 2. Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. (Ref.: Chen, RU et al. (2018). J. Biomed. Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).

Specificity

Clone 164 is a mouse monoclonal antibody that detects NS1 protein of Dengue virus 2. It targets an epitope within the N-terminal half.

Immunogen

Recombinant Dengue Virus 2 NS1 protein.

Application

Quality Control Testing

Evaluated by ELISA with DENV2 NS1 recombinant protein.

ELISA Analysis: 1.0 μg/mL of this antibody detected recombinant Dengue Virus 2 NS1 protein.

Tested Applications

ELISA Analysis: A representative lot detected Dengue Virus 2 NS1 in ELISA applications (Bosch, I., et. al. (2020). PLoS Negl Trop Dis.

14(6):e0008203).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
This mouse monoclonal Anti-Dengue Virus 2 NS1, clone 164, Cat. No. MABF2774, is a mouse monoclonal antibody that detects Dengue virus NS1 protein and is tested for use in ELISA.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


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