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MABF294

Sigma-Aldrich

Anti-LAG3 Antibody, clone 8C7.1

culture supernatant, clone 8C7.1, from mouse

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Synonym(s):
Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223, LAG3
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

8C7.1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LAG3(3902)

Related Categories

General description

Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.

Immunogen

Mouse Fc-tagged recombinant human LAG3 extracellular fragment.

Application

Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.
This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Quality

Evaluated by Western Blotting in NK-92c ell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92 cell lysate.

Target description

~60 kDa observed

Physical form

Mouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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