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MABF3073

Sigma-Aldrich

Anti-SARS-CoV-2 Spike Protein Antibody, clone S3D8

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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

S3D8, monoclonal

mol wt

calculated mol wt 141.18 kDa

purified by

using protein G

species reactivity

SARS coronavirus

packaging

antibody small pack of 100 μg

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
neutralization: suitable

isotype

IgG2a

epitope sequence

Extracellular domain

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

SARS virus ... S(43740568)

Related Categories

General description

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Clone S3D8 exhibits a strong preference for tertiary structure of spike glycoprotein and is not suitable for Western blotting, but grants a remarkable performance in immunoprecipitation. It is shown to block the binding of Spike protein with ACE2 (IC50 = 225.6 ng/mL) and neutralize infection of VeroE6/TM2 cells by SARS-CoV-2 (IC50 = 139 ng/mL). (Ref.: Guo, Y., et al. (2021). J. Biol. Chem. 296; 100346).

Specificity

Clone S3D8 is a mouse monoclonal antibody that detects SARS-CoV-2 spike protein. It recognizes the intact tertiary structure of the spike protein.

Immunogen

Spike TM‐SBP protein (residue 1‐1208 aa) expressed in human Expi293 cells.

Application

Quality Control Testing

Evaluated by ELISA with recombinant SARS-CoV-2 spike protein.

ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant Spike glycoprotein of SARS-CoV-2.

Tested Applications

Immunofluorescence Analysis: A representative lot detected SARS-CoV-2 Spike Protein in Immunofluorescence applications (Guo, Y., et al. (2021). J Biol Chem. 296:100346).

Immunoprecipitation Analysis: A representative lot detected SARS-CoV-2 Spike Protein in Immunoprecipitation applications (Guo, Y., et al. (2021). J Biol Chem. 296:100346).

ELISA Analysis: A representative lot detected SARS-CoV-2 Spike Protein in ELISA applications (Guo, Y., et al. (2021). J Biol Chem. 296:100346).

Neutralizing: A representative lot neutralized SARS-CoV-2 Spike Protein in Neutralizing applications (Guo, Y., et al. (2021). J Biol Chem.;296:100346).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SARS-CoV-2 Spike Protein, clone S3D8, Cat. No. MABF3073, is a mouse monoclonal antibody that detects spike glycoprotein in SARS-CoV-2 and is tested for use in ELISA, Immunofluorescence, Immunoprecipitation, and Neutralizing.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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