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MABF361

Sigma-Aldrich

Anti-CD117 (c-kit) Antibody (mouse), PE, clone ACK2

clone ACK2, 0.2 mg/mL, from rat

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Synonym(s):
Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

PE

antibody form

purified antibody

antibody product type

primary antibodies

clone

ACK2, monoclonal

species reactivity

mouse

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Kit(16590)

Related Categories

General description

The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo. In addition, the antibody is reported to be cross-reactive with rat c-Kit and is extensively published for use in this species.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This rat monoclonal antibody Anti-CD117 (c-kit) (mouse), PE, clone ACK2 validated for use in IHC, ICC & Flow Cytometry for the detection of CD117 (c-kit).

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD117 (c-kit) in one million C57Bl/6 bone marrow cells.

Physical form

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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