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Key Documents

MABN1529

Sigma-Aldrich

Anti-PRICKLE2 Antibody, clone 3B4.1

clone 3B4.1, from mouse

Synonym(s):

Prickle-like protein 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3B4.1, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

General description

Prickle-like protein 2 (UniProt Q7Z3G6) is encoded by the PRICKLE2 (also known as EPM5) gene (Gene ID 166336) in human. Prickle2 is a nuclear membrane associated protein that belongs to a highly conserved Prickle, Espinas, Testin (PET) and Linl-1, Isl-1, and Mec-3 (LIM) domain-containing protein family. Prickle is part of the non-canonical Wnt signaling pathway that establishes planar cell polarity. It plays a critical role in neuron architecture and function. In mice it is shown to be highly expressed in the hippocampus region where it localizes to the post-synaptic density (PSD). It interacts the PSD-95 and NMDA receptor, the two proteins implicated in Autism spectrum disorders. Loss of Prickle2 is known to reduce number and size of miniature synaptic currents, indicating deficits in basal synaptic transmission. Mice deficient in Prickle2 exhibit lower seizure threshold and PRICKLE2 mutations in humans are associated with progressive myoclonic epilepsy type 5. Ref.: Sowers, LP., et al.. (2013), Mol. Psychiatry. 18, 1077-1089.

Specificity

Clone 3B4.1 targets an internal PRICKLE2 epitope conserved between human and murine species.

Immunogen

GST-tagged recombinant human PRICKLE2 internal fragment.

Application

Anti-PRICKLE2 Antibody, clone 3B4.1, Cat. No. MABN1529, is a highly specific mouse monoclonal antibody that targets PRICKLE2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PRICKLE2 in mouse cerebral cortex, as well as in human pancreas, small intestine, and tonsil tissue sections.
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PRICKLE2 in 10 µg of mouse brain tissue lysate.

Target description

~95 kDa observed. 95.62/95.30 kDa (human pro-/mature form) and 95.78/95.47 kDa (mouse pro-/mature form) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yue Ban et al.
Experimental neurology, 347, 113880-113880 (2021-10-02)
Epilepsy and autism spectrum disorders (ASD) frequently show comorbidity, suggesting shared or overlapping neurobiological basis underlying these conditions. R104Q is the first mutation in the PRICKLE 1(PK1) gene that was discovered in human patients with progressive myoclonus epilepsy (PME). Subsequently

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