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MABN1595

Sigma-Aldrich

Anti-Serotonin receptor 2A/HTR2A Antibody, clone 9B11.1

clone 9B11.1, from mouse

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Synonym(s):
5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B11.1, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HTR2A(3356)

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This Item
MAB11041MABN1539SAB4501470
biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

Gene Information

human ... HTR2A(3356)

Gene Information

human ... HTR1A(3350)
rat ... Htr1A(24473)

Gene Information

human ... HTR1B(3351)

Gene Information

human ... HTR1B(3351)

clone

9B11.1, monoclonal

clone

19A9.2, monoclonal

clone

1C2.1, monoclonal

clone

polyclonal

species reactivity

mouse, rat, human

species reactivity

human, rat

species reactivity

human, rat, mouse

species reactivity

human, mouse, rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:5000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100

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General description

5-hydroxytryptamine receptor 2A (UniProt P28223; also known as 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A) is encoded by the HTR2A (also known as HTR2) gene (Gene ID 3356) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-HT2A receptor, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action and contributes to many of the physiological effects of 5-HT. The hallucinogenic activity of LSD is mediated in part by its action as a partial to full agonist of 5-HT2A receptor, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism against 5-HT2A receptor. Human 5-HT2A receptor contains four extracellular domains (a.a. 1-75, 133-148, 216-233, 347-362), seven transmembrane segments (a.a. 76-99, 111-132, 149-171, 192-215, 234-254, 325-346, 363-384), and four cytoplasmic domains (a.a. 100-110, 172-191, 255-324, 385-471).

Specificity

Expected to react with both spliced isoforms of human Serotonin receptor 2A reported by UniProt (P28223).

Immunogen

Epitope: Third cytoplasmic domain.
GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human Serotonin receptor 2A/HTR2A.

Application

Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected 2A/HTR2A in human cerebral cortex, mouse cerebral cortex, and rat testis tissue sections.
Research Category
Neuroscience
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Serotonin receptor 2A/HTR2A Antibody, clone 9B11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of SHTR2A.

Quality

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 2A/HTR2A in 10 µg of human cerebral cortex tissue lysate.

Target description

~52 kDa observed. 52.60 kDa (isoform 1) and 43.94 kDa (isoform 2) calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Teita Asano et al.
British journal of pharmacology, 174(19), 3370-3381 (2017-07-28)
Visceral hypersensitivity is responsible for pathogenesis of irritable bowel syndrome (IBS). Therefore, its prevention can help avoid abdominal pain and discomfort in IBS. To find candidate drugs for visceral hypersensitivity, we screened existing medicines for their ability to prevent visceral
Lizhu Jiang et al.
Frontiers in molecular neuroscience, 12, 317-317 (2020-02-11)
Spaced training is robustly superior to massed training, which is a well-documented phenomenon in humans and animals. However, the mechanisms underlying the spacing effect still remain unclear. We have reported previously that spacing training exerts memory-enhancing effects by inhibiting forgetting
Jung Eun Kim et al.
Science advances, 7(10) (2021-03-05)
Stress is a key risk factor for dystonia, a debilitating motor disorder characterized by cocontractions of muscles leading to abnormal body posture. While the serotonin (5HT) system is known to control emotional responses to stress, its role in dystonia remains

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