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MABN1601

Sigma-Aldrich

Anti-Vitamin D3 Receptor Antibody, clone 6F3.1

clone 6F3.1, from mouse

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Synonym(s):
Vitamin D3 receptor, 1, 25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1, VDR
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6F3.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... VDR (7421)

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MABS2028SAB1406580SAB4503071
species reactivity

human

species reactivity

chicken, rat, porcine, human

species reactivity

human

species reactivity

human

clone

6F3.1, monoclonal

clone

9A7, monoclonal

clone

polyclonal

clone

polyclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Gene Information

human ... VDR (7421)

Gene Information

chicken ... Vdr(395988)
human ... VDR(7421)

Gene Information

human ... VDR(7421)

Gene Information

human ... VDR(7421)

shipped in

ambient

shipped in

-

shipped in

dry ice

shipped in

wet ice

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General description

Vitamin D3 receptor (UniProt P11473; also known as 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1, VDR) is encoded by the VDR (also known as NR1I1) gene (Gene ID 7421) in human. VDR is a member of the superfamily of nuclear receptors for steroid hormones and acts as a ligand-inducible transcription factor that targets vitamin D responsive elements. Upon ligand binding, VDR complexes with the retinoid-X receptor (RXR), the heterodimer then targets hormone-response elements, resulting in transcription or transrepression of specific genes. Ligand binding also induces a conformational change at the C-terminal ligand-binding domain (LBD; a.a. 192-427), resulting in recruitment of the p160/SRC family coactivators whose acetylase activity plays an important role in transcription modulations via chromatin remodeling. VDR gene mutations are associated with type II vitamin D-resistant rickets and alopecia (hair loss).

Specificity

Clone 6F3.1 targets an epitope present at the N-terminal half of both spliced isoforms of human vitamin D3 receptor reported by UniProt (P11473).

Immunogen

GST-tagged recombinant fragment corresponding to the N-terninal of human vitamin D3 receptor.

Application

Anti-Vitamin D3 Receptor Antibody, clone 6F3.1, Cat. No. MABN1601, is a highly specific mouse monoclonal antibody that targets Vitamin D3 receptor and has been tested in Western Blotting.
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected vitamin D3 receptor in 10 µg of T47D human breast cancer cell lysate.

Target description

~50 kDa observed. 48.29/53.88 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified.
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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