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MABN2297

Sigma-Aldrich

Anti-APP Antibody, clone 7H6

clone 7H6, from rat

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Synonym(s):
Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II
eCl@ss:
32160702

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

7H6, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... APP(351)

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This Item
MABN2287MABN852ABN1643
Anti-APP Antibody, clone 7H6 clone 7H6, from rat

MABN2297

Anti-APP Antibody, clone 7H6

Anti-APP Antibody, clone 1D1 clone 1D1, from rat

MABN2287

Anti-APP Antibody, clone 1D1

Anti-APP Antibody, clone 2B3 clone 2B3, from mouse

MABN852

Anti-APP Antibody, clone 2B3

biological source

rat

biological source

rat

biological source

mouse

biological source

rabbit

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

clone

7H6, monoclonal

clone

1D1, monoclonal

clone

2B3, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

mouse, human

species reactivity

human, mouse

UniProt accession no.

P05067

UniProt accession no.

P05067

UniProt accession no.

P05067

UniProt accession no.

P05067

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General description

Amyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer′s disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury.

Specificity

Clone 7H6 specifically detects human amyloid precursor protein (APP) and does not display reactivity with other species. It targets an epitope within the ectodomain region of APP.

Immunogen

StrepII-tagged human recombinant ectodomain region of the neuronal isoform hAPP695 lacking the KPI domain.

Application

Anti-APP, clone 7H6, Cat. No. MABN2297, is a highly specific rat monoclonal antibody, that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected APP in HEK293 cells.

Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Flow Cytometry Analysis: A representative lot detected APP in Flow Cytometry applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Western Blotting Analysis: A 1:10 dilution from a representative lot detected App in WT and APP knockdown HEK293T cells (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

Immunoprecipitation Analysis: A representative lot detected APP in Immunoprecipitation applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

ELISA Analysis: A representative lot detected APP in ELISA applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected APP in HEK293T either lentivirally transduced with APP shRNA-1 or APP shRNA-2 both coexpressing GFP as a reporter (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Hoefling, C., et. al. (2016). Aging Cell. 15(5):953-63).
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected APP in 10 µg of HEK293 cell lysate.

Target description

~95 kDa observed; 86.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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