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MABN690

Sigma-Aldrich

Anti-PPP1CA Antibody, clone 5E9

ascites fluid, clone 5E9, from mouse

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Synonym(s):
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5E9, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PPP1CA(5499)

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This Item
SAB5700456P7607PLA0154
Anti-PPP1CA Antibody, clone 5E9 ascites fluid, clone 5E9, from mouse

MABN690

Anti-PPP1CA Antibody, clone 5E9

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

affinity purified immunoglobulin

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

rabbit, rat, bovine, human, mouse, monkey

species reactivity

human, mouse

clone

5E9, monoclonal

clone

polyclonal

clone

PPI-377, monoclonal

clone

-

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:100, western blot: 1:500-1:2000

technique(s)

immunocytochemistry: suitable, microarray: suitable, western blot: 1:500 using mouse fibroblasts cell extract

technique(s)

immunohistochemistry: 1:500- 1:2,000, western blot: 1:2,000- 1:10,000

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General description

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP-1A) encoded by the gene named PPP1CA or PPP1A is one of the three catalytic subunits of protein phosphatase 1 (PP1), one of the major protein phosphatases in the cell. PPP1CA interacts with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate a huge variety of target proteins. PPP1CA is critical for cell division, chromatin structure, protein synthesis, glycogen metabolism, muscle and cardiac function as well as the regulation of long-term ionic conductances and even synaptic plasticity. Developmentally it has nearly as many roles with principal ones being as a regulator of neural tube and optic fissure closure as well as enteric neural crest cell (ENCCs) migration during development. PPP1CA can be found both cytoplasmically and nuclear localized depending upon its regulatory partner but PPP1CA is mostly nuclear/nucleoplasm localized. Interestingly in the brain, PPP1CA also functions as a suppressor of learning and memory and as a potential mediator of cognitive decline during aging. EMD-Millipore’s Anti-PPP1CA mouse monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HeLa, HepG2, MCF-7, Jurkat and A549 cells and by direct ELISA against varying concentrations of antigen.

Immunogen

Purified recombinant fragment of human PPP1CA expressed in E. Coli.

Application

Anti-PPP1CA Antibody, clone 5E9 is a highly specific mouse monoclonal antibody & that targets Serine/threonine-protein phosphatase & has been tested in western blotting.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPP1CA in HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates.

Optimal working dilutions must be determined by end user.

Target description

~38 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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