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MABN875

Sigma-Aldrich

Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7

clone KC4-8A7, from mouse

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Synonym(s):
Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, GABAA γ2 Subunit
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

KC4-8A7, monoclonal

species reactivity

rat, bovine, human, mouse

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GABRG2(2566)

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This Item
MABN263MABN1724SAB4501071
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

KC4-8A7, monoclonal

clone

10F10-C1-B8, monoclonal

clone

N399/19, monoclonal

clone

polyclonal

species reactivity

rat, bovine, human, mouse

species reactivity

rat

species reactivity

rat, human, mouse

species reactivity

rat, human, mouse

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

isotype

IgG2aκ

isotype

IgG2bκ

isotype

IgG1κ

isotype

-

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General description

Gamma-aminobutyric acid (GABA) receptor subunit gamma-2 (UniProt P18507; also known as GABA(A) receptor subunit gamma-2) is encoded by the GABRG2 (also known as CAE2, ECA2, GEFSP3) gene (Gene ID 2566) in human. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Upon GABA binding and activation, the GABAA receptor selectively conducts chloride ions through its pore, resulting in hyperpolarization of the neuron. The GABAAR is a pentameric complex composed of two alpha, two beta, and one gamma subunit arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The gamma 2 subunit is initially produced with an signal peptide sequence (a.a. 1-39), which is then cleaved off to yield the mature subunit (a.a. 40 – 467).

Specificity

Expected to react with all three spliced isoforms of GABA(A) receptor gamma 2 subunit (GABRG2), but not gamma 1 (GABRG1) or gamma 3 (GABRG3) subunit.

Immunogen

Epitope: cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human GABAA γ2 Subunit.

Application

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, ELISA for the detection of GABAA.
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GABAA γ2 Subunit in human cerebral cortex tissue.
Western Blotting Analysis: A representative lot detected human GABAA gamma2 subunit cytoplasmic domain GST fusion protein, as well as endogenous GABAA gamma2 subunit in purified bovine brain GABAA receptor complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABAA receptor complex from rat cerebral cortex membrane extracts, as well as purified bovine brain GABAA receptor complex as measured by ligand-binding activity associated with the immune complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).
ELISA Analysis: A representative lot detected human GABAA gamma2 cytoplasmic domain GST fusion protein, but not GST (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GABAA γ2 Subunit in 10 µg of rat brain tissue lysate.
Note: For Western blotting analysis, DO NOT BOIL samples prior to electrophoresis. After dissociating receptor complex with SDS sample buffer, remove non-solublized material by centrifugation.

Target description

~45 kDa observed. Molecular weight bands were observed at ~45 kDa for both rat and mouse brain tissue lysates. However, for human brain tissue lysates, we detected a molecular weight at ~58 kDa, as predicted by Uniprot.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Gloria S Forkuo et al.
Molecular pharmaceutics, 14(6), 2088-2098 (2017-04-26)
We describe pharmacokinetic and pharmacodynamic properties of two novel oral drug candidates for asthma. Phenolic α4β3γ2 GABAAR selective compound 1 and acidic α5β3γ2 selective GABAAR positive allosteric modulator compound 2 relaxed airway smooth muscle ex vivo and attenuated airway hyperresponsiveness
Abdeslam Mouihate et al.
CNS neuroscience & therapeutics, 26(2), 240-250 (2019-07-25)
Experimental studies have shown that the progesterone metabolite, allopregnanolone, is endowed with promyelinating effects. The mechanisms underlying these promyelinating effects are not well understood. Therefore, we explored the impact of allopregnanolone's synthetic analogue, ganaxolone, on remyelination and microglial activation following
Prabhuanand Selvaraj et al.
Cells, 10(12) (2021-12-25)
Modulation of the endocannabinoid system has emerged as an effective approach for the treatment of many neurodegenerative and neuropsychological diseases. However, the underlying mechanisms are still uncertain. Using a repetitive mild traumatic brain injury (mTBI) mouse model, we found that

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