MilliporeSigma
All Photos(2)

Documents

MABS1136

Sigma-Aldrich

Anti-Cathelicidin/FALL-39/LL-37 Antibody, clone 8C10.1

clone 8C10.1, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Cathelicidin antimicrobial peptide, 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

8C10.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CAMP(820)

General description

Cathelicidin antimicrobial peptide (UniProt P49913; also known as 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18) is encoded by the CAMP (also known as CAP18, FALL39, LL37) gene (ORF HSD26; Gene ID 820) in human. The human cathelicidin antimicrobial protein 18 (hCAP-18) is initially translated as a precursor molecule with a signal peptide (a.a. 1-30) and a propeptide (a.a. 31-131) sequence, the removal of which yields the mature hCAP-18 (a.a. a.a. 132-173). During the inflammatory response, hCAP-18 is proteolytically cleaved into the FALL-39 (a.a 132-170), which is further processed to yield the antimicrobial protein LL-37 (CAP-37; a.a. 134-170). Neutrophils store preformed hCAP-18 within specific granules and release it into inflamed tissue as a result of granule exocytosis. Once released, hCAP-18 is activated by proteinase-3-mediated cleavage, a component of neutrophil primary granules. In addition to its microbicidal and LPS-sequestering activity, LL-37 is also involved in the regulation of innate and humoral immune responses, wound healing, and tissue repair.

Specificity

Clone 8C10.1 targets a C-terminal epitope present in the prepro- (a.a. 1-173) and pro- (a.a. 31-173) forms of hCAP-18, as well as the processed antibacterial peptides FALL-39 (a.a 132-170) and LL-37 (a.a. 134-170), but not the preprodomain (a.a. 1-131) or prodomain (a.a. 31-131) fragment.

Immunogen

Epitope: C-terminal.
KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Cathelicidin/FALL-39/LL-37.

Application

Anti-Cathelicidin Antibody, clone 8C10.1 is an antibody against Cathelicidin/FALL-39/LL-37 for use in Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cathelicidin/FALL-39/LL-37 immunoreactivity in human spleen tissue sections.
Research Category
Signaling
Research Sub Category
Inflammation & Autoimmune Mechanisms

Quality

Evaluated by Immunohistochemistry in human bone marrow tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cathelicidin/FALL-39/LL-37 immunoreactivity in human bone marrow tissue sections.

Target description

19.30 kDa (prepro-hCAP-18), 16.02 kDa (pro-hCAP-18), 4.712 kDa (FALL-39; a.a. 132-170) and 4.493 kDa (LL-37; a.a. 134-170) calculated.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service