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MABS2195

Sigma-Aldrich

Anti-gp91phox (NOX2) Antibody

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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

54.1, monoclonal

mol wt

calculated mol wt 65.34 kDa
observed mol wt ~91 kDa

species reactivity

human

packaging

antibody small pack of 100 μg

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

dry ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CYBB(1536)

Related Categories

General description

Cytochrome b-245 heavy chain (UniProt: P04839; also known as CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome) is encoded by the CYBB (also known as NOX2) gene (Gene ID: 1536) in Human. Gp91phox is a multi-pass membrane glycoprotein of the NADPH oxidase family that contains three extracellular domains, six transmembrane domains, and four cytoplasmic domains. It contains four iron binding sites and has a ferric oxidoreductase domain that is localized in the amino acids 54-286. It is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide and plays an important role in the host defense function of phagocytic cells. It also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It can undergo polyubiquitination at lysine 48 by RNF145 that triggers its endoplasmic reticulum-associated degradation. Clone 54.1 recognizes the conserved carboxy-terminal domain of NOX family proteins and shows higher specificity for NOX2 and reduced reactivity with NOX3, NOX4, and NOX1 in the descending order. Mutations in CYBB gene are known to cause granulomatous disease that is characterized by the inability of neutrophils and phagocytes to kill ingested leading to life-threatening bacterial and fungal infections. (Ref.: (Ref.: Burritt, JB., et al. (2003). J. Immunol. 170 (12); 6082-6089).

Specificity

Clone 54.1 is a mouse monoclonal antibody that detects human gp91phox/NOX2. It targets an epitope within 8 amino acids from the C-terminal, fourth cytoplasmic domain.

Immunogen

Partially purified human neutrophil flavocytochrome b588 ( gp91-phox).

Application

Quality Control Testing

Evaluated by Western Blotting in Human PMN cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected gp91phox (NOX2) in Human PMN cell lysate.

Tested Applications


Western Blotting Analysis: A representative lot detected gp91phox (NOX2) in Western Blotting applications (Baniulis, D., et. al. (2005). Biochim Biophys Acta. 1752(2):186-96; Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

Flow Cytometry Analysis: A representative lot detected gp91phox (NOX2) in Flow Cytometry applications (Burritt, J.B., et. al. (2003). J Immunol. 170(12):6082-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-gp91phox (NOX2) clone 54.1, Cat. No. MABS2195, is a mouse monoclonal antibody that detects Cytochrome b-245 heavy chain ( gp91phox) and is tested for use in Flow Cytometry and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


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